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Microsatellite loci in the tiger shark and cross-species amplification using pyrosequencing technology

dc.contributor.authorMendes, Natalia J.
dc.contributor.authorCruz, Vanessa P.
dc.contributor.authorAshikaga, Fernando Y.
dc.contributor.authorCamargo, Samia M.
dc.contributor.authorOliveira, Claudio
dc.contributor.authorPiercy, Andrew N.
dc.contributor.authorBurgess, George H.
dc.contributor.authorCoelho, Rui
dc.contributor.authorSantos, Miguel N.
dc.contributor.authorMendonca, Fernando F.
dc.contributor.authorForesti, Fausto
dc.date.accessioned2017-04-07T15:56:10Z
dc.date.available2017-04-07T15:56:10Z
dc.date.issued2016-08
dc.description.abstractThe tiger shark (Galeocerdo cuvier) has a global distribution in tropical and warm temperate seas, and it is caught in numerous fisheries worldwide, mainly as bycatch. It is currently assessed as near threatened by the International Union for Conservation of Nature (IUCN) Red List. In this study, we identified nine microsatellite loci through next generation sequencing (454 pyrosequencing) using 29 samples from the western Atlantic. The genetic diversity of these loci were assessed and revealed a total of 48 alleles ranging from 3 to 7 alleles per locus (average of 5.3 alleles). Cross-species amplification was successful at most loci for other species such as Carcharhinus longimanus, C. acronotus and Alopias superciliosus. Given the potential applicability of genetic markers for biological conservation, these data may contribute to the population assessment of this and other species of sharks worldwide.
dc.identifier.doi10.7717/peerj.2205
dc.identifier.issn2167-8359
dc.identifier.otherAUT: RMS01092;
dc.identifier.urihttp://hdl.handle.net/10400.1/9335
dc.language.isoeng
dc.peerreviewedyes
dc.relation.isbasedonWOS:000383262700001
dc.titleMicrosatellite loci in the tiger shark and cross-species amplification using pyrosequencing technology
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage2205
oaire.citation.startPage2205
oaire.citation.titlePEERJ
oaire.citation.volume4
person.familyNameCoelho
person.givenNameRui
person.identifier134638
person.identifier.ciencia-idDC1A-20B7-EAC0
person.identifier.orcid0000-0003-3813-5157
person.identifier.ridC-1163-2008
person.identifier.scopus-author-id56257111100
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication6366aa1b-1301-43a3-b2b0-1280311ac95f
relation.isAuthorOfPublication.latestForDiscovery6366aa1b-1301-43a3-b2b0-1280311ac95f

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