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Protein changes as robust signatures of fish chronic stress: a proteomics approach to fish welfare research

2020-04-19Journal article Open access
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dc.contributor.authorRaposo de Magalhães, Cláudia
dc.contributor.authorSchrama, Denise
dc.contributor.authorFarinha, Ana Paula
dc.contributor.authorRevets, Dominique
dc.contributor.authorKuehn, Annette
dc.contributor.authorPlanchon, Sébastien
dc.contributor.authorRodrigues, Pedro
dc.contributor.authorCerqueira, Marco
dc.date.accessioned2020-05-08T14:00:10Z
dc.date.available2020-05-08T14:00:10Z
dc.date.issued2020-04-19
dc.date.updated2020-05-01T03:31:53Z
dc.description.abstractBackground Aquaculture is a fast-growing industry and therefore welfare and environmental impact have become of utmost importance. Preventing stress associated to common aquaculture practices and optimizing the fish stress response by quantification of the stress level, are important steps towards the improvement of welfare standards. Stress is characterized by a cascade of physiological responses that, in-turn, induce further changes at the whole-animal level. These can either increase fitness or impair welfare. Nevertheless, monitorization of this dynamic process has, up until now, relied on indicators that are only a snapshot of the stress level experienced. Promising technological tools, such as proteomics, allow an unbiased approach for the discovery of potential biomarkers for stress monitoring. Within this scope, using Gilthead seabream (Sparus aurata) as a model, three chronic stress conditions, namely overcrowding, handling and hypoxia, were employed to evaluate the potential of the fish protein-based adaptations as reliable signatures of chronic stress, in contrast with the commonly used hormonal and metabolic indicators. Results A broad spectrum of biological variation regarding cortisol and glucose levels was observed, the values of which rose higher in net-handled fish. In this sense, a potential pattern of stressor-specificity was clear, as the level of response varied markedly between a persistent (crowding) and a repetitive stressor (handling). Gel-based proteomics analysis of the plasma proteome also revealed that net-handled fish had the highest number of differential proteins, compared to the other trials. Mass spectrometric analysis, followed by gene ontology enrichment and protein-protein interaction analyses, characterized those as humoral components of the innate immune system and key elements of the response to stimulus. Conclusions Overall, this study represents the first screening of more reliable signatures of physiological adaptation to chronic stress in fish, allowing the future development of novel biomarker models to monitor fish welfare.pt_PT
dc.description.sponsorshipThis study received Portuguese national funds from FCT - Foundation for Science and Technology through project UIDB/04326/2020 and project WELFISH (Refª 16–02-05-FMP-12, “Establishment of Welfare Biomarkers in farmed fish using a proteomics approach”) financed by Mar2020, in the framework of the program Portugal 2020. Cláudia Raposo de Magalhães acknowledges an FCT PhD scholarship, Refª SFRH/BD/138884/2018. Denise Schrama acknowledges an FCT PhD scholarship, Refª SFRH/BD/136319/2018.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBMC Genomics. 2020 Apr 19;21(1):309pt_PT
dc.identifier.dois12864-020-6728-4pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.1/13859
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherBMCpt_PT
dc.rights.holderThe Author(s).
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAquaculturept_PT
dc.subjectBiomarkerspt_PT
dc.subjectCortisolpt_PT
dc.subjectGlucosept_PT
dc.subjectProteomept_PT
dc.subjectMass-spectrometrypt_PT
dc.titleProtein changes as robust signatures of fish chronic stress: a proteomics approach to fish welfare researchpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue1pt_PT
oaire.citation.startPage309pt_PT
oaire.citation.titleBMC Genomicspt_PT
oaire.citation.volume12pt_PT
person.familyNameRaposo de Magalhães
person.familyNameSchrama
person.familyNameMARTINS FARINHA RESENDE
person.familyNameRodrigues
person.familyNameCerqueira
person.givenNameCláudia
person.givenNameDenise
person.givenNameANA PAULA
person.givenNamePedro
person.givenNameMarco
person.identifier159154
person.identifierG-5692-2017
person.identifier.ciencia-idEB1C-0E40-F003
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person.identifier.ciencia-id4312-4B8F-3079
person.identifier.ciencia-id4F1E-16FC-00D8
person.identifier.orcid0000-0001-7751-2870
person.identifier.orcid0000-0003-0274-5957
person.identifier.orcid0000-0003-1455-5958
person.identifier.orcid0000-0002-9668-1204
person.identifier.orcid0000-0001-7237-5053
person.identifier.ridH-6511-2016
person.identifier.ridM-3406-2013
person.identifier.scopus-author-id55755583400
person.identifier.scopus-author-id54386717500
person.identifier.scopus-author-id55107531000
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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