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Unlocking the in vitro anti-Trypanosoma cruzi activity of halophyte plants from the southern Portugal

2016-08Journal article Open access
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dc.contributor.authorOliveira, Marta
dc.contributor.authorSales Junior, Policarpo Ademar
dc.contributor.authorRodrigues, Maria Joao
dc.contributor.authorDellaGreca, Marina
dc.contributor.authorBarreira, Luísa
dc.contributor.authorFonseca Murta, Silvane Maria
dc.contributor.authorRomanha, Álvaro José
dc.contributor.authorCustódio, Luísa
dc.date.accessioned2017-04-07T15:56:15Z
dc.date.available2017-04-07T15:56:15Z
dc.date.issued2016-08
dc.description.abstractObjective: To evaluate the in vitro anti-Trypanosoma cruzi (T. cruzi) activity of organic extracts prepared from halophyte species collected in the southern coast of Portugal (Algarve), and chemically characterize the most active samples.Methods: Acetone, dichloromethane and methanol extracts were prepared from 31 halophyte species and tested in vitro against trypomastigotes and intracellular amastigotes of the Tulahuen strain of T. cruzi. The most active extract was fractionated by preparative HPLC-DAD, affording 11 fractions. The most selective fraction was fully characterized by H-1 NMR.Results: From 94 samples tested, one was active, namely the root dichloromethane extract of Juncus acutus (IC50 < 20 mu g/mL). This extract was fractionated by HPLC, affording 11 fractions, one of them containing only a pure compound (juncunol), and tested for anti-parasitic activity. Fraction 8 (IC50 = 4.1 mu g/mL) was the most active, and was further characterized by H-1 NMR. The major compounds were phenanthrenes, 9,10-dihydrophenanthrenes and benzocoumarins.Conclusion: Our results suggest that the compounds identified in fraction 8 are likely responsible for the observed anti parasitic activity. Further research is in progress aiming to isolate and identify the specific active molecules. To the best of our knowledge, this is the first report on the in vitro anti T. cruzi activity of halophyte species.
dc.description.sponsorshipPROEP/CNPq/FIOCRUZ 401988/2012-0; FCT/CAPES 2358, 2014/2015
dc.identifier.doi10.1016/j.apjtm.2016.06.015
dc.identifier.issn1995-7645
dc.identifier.otherAUT: LBA00541;
dc.identifier.urihttp://hdl.handle.net/10400.1/9361
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationHalophytes: a precious resource of nutritional elements and bioactive compounds
dc.relationBiologically Active Natural Products from Marine Organisms and Salt Marsh Plants (acronym: PharmaBlue)
dc.relation.isbasedonWOS:000383242400003
dc.subjectChagas disease
dc.subjectHalophytes
dc.subjectTrypanosoma cruzi
dc.subjectPhenanthrenes
dc.subjectJuncunol
dc.titleUnlocking the in vitro anti-Trypanosoma cruzi activity of halophyte plants from the southern Portugal
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHalophytes: a precious resource of nutritional elements and bioactive compounds
oaire.awardTitleBiologically Active Natural Products from Marine Organisms and Salt Marsh Plants (acronym: PharmaBlue)
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR-EST%2F4346%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00049%2F2012%2FCP0179%2FCT0001/PT
oaire.citation.endPage741
oaire.citation.issue8
oaire.citation.startPage735
oaire.citation.titleAsian Pacific Journal of Tropical Medicine
oaire.citation.volume9
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
oaire.fundingStreamInvestigador FCT
person.familyNameOliveira
person.familyNameRodrigues
person.familyNameBarreira
person.familyNameCustódio
person.givenNameMarta
person.givenNameMaria João
person.givenNameLuísa
person.givenNameLuísa
person.identifierR-004-VNG
person.identifier.ciencia-idC618-E728-0B4C
person.identifier.ciencia-id2514-0E17-1D8D
person.identifier.ciencia-id6D1A-17E5-1400
person.identifier.ciencia-id791B-C560-AEA2
person.identifier.orcid0000-0001-6793-8026
person.identifier.orcid0000-0001-8732-710X
person.identifier.orcid0000-0002-4077-855X
person.identifier.orcid0000-0003-4338-7703
person.identifier.ridM-6101-2013
person.identifier.scopus-author-id56031608100
person.identifier.scopus-author-id15831018900
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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