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Hypoxic induced decrease in oxygen consumption in Cuttlefish (Sepia officinalis) is associated with minor increases in mantle octopine but no changes in markers of protein turnover

2017Journal article Open access
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dc.contributor.authorCapaz, Juan Carlos
dc.contributor.authorTunnah, Louise
dc.contributor.authorMacCormack, Tyson J.
dc.contributor.authorLamarre, Simon G.
dc.contributor.authorSykes, António V.
dc.contributor.authorDriedzic, William R.
dc.date.accessioned2018-09-27T14:58:43Z
dc.date.available2018-09-27T14:58:43Z
dc.date.issued2017
dc.description.abstractThe common cuttlefish (Sepia officinalis), a dominant species in the north-east Atlantic ocean and Mediterranean Sea, is potentially subject to hypoxic conditions due to eutrophication of coastal waters and intensive aquaculture. Here we initiate studies on the biochemical response to an anticipated level of hypoxia. Cuttlefish challenged for 1 h at an oxygen level of 50% dissolved oxygen saturation showed a decrease in oxygen consumption of 37% associated with an 85% increase in ventilation rate. Octopine levels were increased to a small but significant level in mantle, whereas there was no change in gill or heart. There were no changes in mantle free glucose or glycogen levels. Similarly, the hypoxic period did not result in changes in HSP70 or polyubiquinated protein levels in mantle, gill, or heart. As such, it appears that although there was a decrease in metabolic rate there was only a minor increase in anaerobic metabolism as evidenced by octopine accumulation and no biochemical changes that are hallmarks of alterations in protein trafficking. Experiments with isolated preparations of mantle, gill, and heart revealed that pharmacological inhibition of protein synthesis could decrease oxygen consumption by 32 to 42% or Na+/K+ ATPase activity by 24 to 54% dependent upon tissue type. We propose that the decrease in whole animal oxygen consumption was potentially the result of controlled decreases in the energy demanding processes of both protein synthesis and Na+/K+ ATPase activity.pt_PT
dc.description.sponsorshipAction FA1301
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3389/fphys.2017.00344pt_PT
dc.identifier.othercorecção do artigo http://hdl.handle.net/10400.1/12443
dc.identifier.urihttp://hdl.handle.net/10400.1/10858
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontiers Mediapt_PT
dc.relationTowards cephalopod aquaculture: cuttlefish as the spearhead species
dc.subjectEuropean cuttlefishpt_PT
dc.subjectSepia officinalispt_PT
dc.subjectHSP70pt_PT
dc.subjectOctopinept_PT
dc.subjectPolyubiquitinated proteinpt_PT
dc.subjectVentilation frequencypt_PT
dc.titleHypoxic induced decrease in oxygen consumption in Cuttlefish (Sepia officinalis) is associated with minor increases in mantle octopine but no changes in markers of protein turnoverpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTowards cephalopod aquaculture: cuttlefish as the spearhead species
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00576%2F2014%2FCP1217%2FCT0002/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.citation.startPage344pt_PT
oaire.citation.titleFrontiers in Physiologypt_PT
oaire.citation.volume8pt_PT
oaire.fundingStreamInvestigador FCT
oaire.fundingStream5876
person.familyNameCapaz
person.familyNameSykes
person.givenNameJuan Carlos
person.givenNameAntónio
person.identifier107454
person.identifier.ciencia-id5817-4C54-7E98
person.identifier.ciencia-id7510-6641-5A42
person.identifier.orcid0000-0003-4682-5756
person.identifier.orcid0000-0002-5207-0612
person.identifier.ridC-3609-2012
person.identifier.scopus-author-id57194230202
person.identifier.scopus-author-id7102626426
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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