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Identification of RAPD markers linked to the Fin and P loci in Phaseolus vulgaris L.

2009Journal article Restricted access
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dc.contributor.authorApostolova, E.
dc.contributor.authorSvetleva, D.
dc.contributor.authorCarlier, Jorge
dc.contributor.authorLeitão, José
dc.date.accessioned2015-06-19T14:36:26Z
dc.date.available2015-06-19T14:36:26Z
dc.date.issued2009
dc.description.abstractBulked segregant analysis was used to identify random amplified polymorphic DNA RAPD markers linked to the genes for indeterminate growth habit (Fin) and seed color (P) in common bean (Phaseolus vulgaris L.) using an F2 population derived from the cross A 195 × Kristal 137. The variety A 195 is characterized by determinate growth habit and brown colored seeds, while Kristal 137 exhibits indeterminate growth habit and white colored seeds. Among the 140 RAPD primers used to discriminate between two parental genotypes and between two bulks of DNA, respectively, from determinate and indeterminate plants, only 64 (45.71%) amplified polymorphic markers. Nineteen primers amplified clear polymorphic bands and were further tested among 100 F 2 plants. The analysis of the genetic linkage between analyzed molecular markers and the loci for indeterminate growth habit and seed color was performed using the program Join Map 3.0. The logarithm of odds (LOD) of 8.0 and 10 were used as thresholds for linkage grouping, respectively of locus Fin and locus P. One RAPD marker, AA18-600-P1, was found linked to the Fin locus that determines plant habit type and mapped at 17.6 cM apart from it (rec. freq. = 0.162). Five RAPD markers were found linked to the P locus, with distances to this locus varying from 4.1 cM (marker AB18 1500-P2, rec. freq.= 0.034) to 42.6 cM (marker Q03700-P1).
dc.identifier.isbn9789066055520
dc.identifier.issn0567-7572
dc.identifier.otherAUT: JLE00446
dc.identifier.urihttp://hdl.handle.net/10400.1/6502
dc.language.isoeng
dc.peerreviewedyes
dc.relation.isbasedonP-007-SB6
dc.titleIdentification of RAPD markers linked to the Fin and P loci in Phaseolus vulgaris L.
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage106
oaire.citation.startPage101
oaire.citation.titleActa Horticulturae
oaire.citation.volume830
person.familyNameCarlier
person.familyNameLeitão
person.givenNameJorge
person.givenNameJosé
person.identifier258398
person.identifier.ciencia-id691D-A1BB-15CC
person.identifier.ciencia-id7A17-3E0A-73AD
person.identifier.orcid0000-0003-0675-2716
person.identifier.orcid0000-0003-4278-0415
person.identifier.ridAAH-3958-2020
person.identifier.scopus-author-id7102166949
person.identifier.scopus-author-id7005119796
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublicationd4af2b00-61d6-4fd9-b05d-9ed235b5c7de
relation.isAuthorOfPublication0f990d1c-2913-4f8a-8fb6-f8b02721247f
relation.isAuthorOfPublication.latestForDiscoveryd4af2b00-61d6-4fd9-b05d-9ed235b5c7de

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