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Development of an ecotoxicological protocol for the deep-sea fauna using the hydrothermal vent shrimp Rimicaris exoculata

dc.contributor.authorAuguste, M.
dc.contributor.authorMestre, N. C.
dc.contributor.authorRocha, T. L.
dc.contributor.authorCardoso, C.
dc.contributor.authorCueff-Gauchard, V.
dc.contributor.authorLe Bloa, S.
dc.contributor.authorCambon-Bonavita, M. A.
dc.contributor.authorShillito, B.
dc.contributor.authorZbinden, M.
dc.contributor.authorRavaux, J.
dc.contributor.authorBebianno, Maria João
dc.date.accessioned2016-12-16T15:15:49Z
dc.date.available2019-01-01T01:30:10Z
dc.date.issued2016-06
dc.description.abstractIn light of deep-sea mining industry development, particularly interested in massive-sulphide deposits enriched in metals with high commercial value, efforts are increasing to better understand potential environmental impacts to local fauna. The aim of this study was to assess the natural background levels of biomarkers in the hydrothermal vent shrimp Rimicaris exoculata and their responses to copper exposure at in situ pressure (30MPa) as well as the effects of depressurization and pressurization of the high-pressure aquarium IPOCAMP. R. exoculata were collected from the chimney walls of the hydrothermal vent site TAG (Mid Atlantic Ridge) at 3630m depth during the BICOSE cruise in 2014. Tissue metal accumulation was quantified in different tissues (gills, hepatopancreas and muscle) and a battery of biomarkers was measured: metal exposure (metallothioneins), oxidative stress (catalase, superoxide dismutase, glutathione-S-transferase and glutathione peroxidase) and oxidative damage (lipid peroxidation). Data show a higher concentration of Cu in the hepatopancreas and a slight increase in the gills after incubations (for both exposed groups). Significant induction of metallothioneins was observed in the gills of shrimps exposed to 4μM of Cu compared to the control group. Moreover, activities of enzymes were detected for the in situ group, showing a background protection against metal toxicity. Results suggest that the proposed method, including a physiologically critical step of pressurizing and depressurizing the test chamber to enable the seawater exchange during exposure to contaminants, is not affecting metal accumulation and biomarkers response and may prove a useful method to assess toxicity of contaminants in deep-sea species.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.aquatox.2016.03.024pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.1/8851
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationManaging Impacts of Deep-seA reSource exploitation
dc.rights.urihttp://creativecommons.org/licenses/by-nd/4.0/pt_PT
dc.subjectDeep-sea miningpt_PT
dc.subjectHydrothermal ventspt_PT
dc.subjectBiomarkerspt_PT
dc.subjectIpocamppt_PT
dc.subjectCopperpt_PT
dc.subjectTAGpt_PT
dc.titleDevelopment of an ecotoxicological protocol for the deep-sea fauna using the hydrothermal vent shrimp Rimicaris exoculatapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleManaging Impacts of Deep-seA reSource exploitation
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/603418/EU
oaire.citation.endPage285pt_PT
oaire.citation.startPage277pt_PT
oaire.citation.titleAquatic Toxicologypt_PT
oaire.citation.volume175pt_PT
oaire.fundingStreamFP7
person.familyNameBebianno
person.givenNameMaria
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0003-1492-8566
person.identifier.scopus-author-id7004152715
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isAuthorOfPublication.latestForDiscovery2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isProjectOfPublication35ab4469-7888-46b5-ba82-a7bec3e73077
relation.isProjectOfPublication.latestForDiscovery35ab4469-7888-46b5-ba82-a7bec3e73077

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