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Development and application of a SCAR marker to monitor and quantify populations of the postharvest biocontrol agent Pantoea agglomerans CPA-2

2008-03Journal article Open access
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dc.contributor.authorNunes, Carla
dc.contributor.authorBajji, Mohammed
dc.contributor.authorStepien, Valerie
dc.contributor.authorManso, Teresa
dc.contributor.authorTorres, Rosario
dc.contributor.authorUsall, Josep
dc.contributor.authorJijakli, M. Haissam
dc.date.accessioned2018-12-07T14:53:45Z
dc.date.available2018-12-07T14:53:45Z
dc.date.issued2008-03
dc.description.abstractPantoea agglomerans CPA-2 is an effective biocontrol agent of postharvest diseases of citrus and pome fruit. A monitoring technique was developed for its identification and to quantify its populations. The methodology used consisted of (i) searching for a semi-selective medium, (ii) identification of molecular markers and (iii) monitoring population dynamics in a commercial trial. As a semi-selective medium, Malonate Broth Agar supplemented with tetracycline hydroxychloricle and incubation at high temperature (max. of 40 degrees C) facilitated the selective recovery of P agglomerans CPA-2 colonies. The RAPD technique was applied to a collection of 13 strains of P. agglomerans, including CPA-2. Among the 12 primers tested, OPL-11 amplified a fragment (about 720 bp) specific to strain CPA-2. On the basis of this fragment, two SCAR markers were amplified using a primer pair derived from OPL-11 elongation. A first SCAR marker of 720 bp was specifically amplified for the strain CPA-2 and a second one of 270bp was obtained for all P. agglomerans strains tested, including CPA-2. Commercial trials demonstrated a significant reduction of decay with the treatment of formulated cells of R agglomerans CPA-2. Population dynamics of CPA-2 in commercial trials were determined on fruit surfaces and in the environment using both the classical plating technique and PCR with SCAR primers. In general, no significant differences were observed between results obtained from the two methods. On fruit surfaces, 1 day after CPA-2 applied its population by classical methods was 4.37 x 10(6) cfu wound(-1) and at the end of the experiment the population increased to 5.8 x 10(5) cfu wound(-1). The percentages of colonies identified as P agglomerans CPA-2 at these sampling times using SCAR primers were 90 and 95%, respectively. Population dynamics in the environment to evaluate the environmental fate of R agglomerans CPA-2 showed that it has a limited persistence and limited capacity for dispersion. (c) 2007 Published by Elsevier B.V.
dc.identifier.doi10.1016/j.postharvbio.2007.07.016
dc.identifier.issn0925-5214
dc.identifier.urihttp://hdl.handle.net/10400.1/11669
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier Science Bv
dc.subjectReal-Time Pcr
dc.subjectBiological-Control Agent
dc.subjectCandida-Sake Cpa-1
dc.subjectAnomala Strain-K
dc.subjectAureobasidium-Pullulans
dc.subjectApple Fruit
dc.subjectBlue Mold
dc.subjectCombination
dc.subjectDiseases
dc.subjectViability
dc.titleDevelopment and application of a SCAR marker to monitor and quantify populations of the postharvest biocontrol agent Pantoea agglomerans CPA-2
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage428
oaire.citation.issue3
oaire.citation.startPage422
oaire.citation.titlePostharvest Biology and Technology
oaire.citation.volume47
rcaap.rightsopenAccess
rcaap.typearticle

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