Publication
Oxidative stress in toadfish (Halobactrachus didactylus) cardiac muscle Acute exposure to vanadate oligomers
| dc.contributor.author | Aureliano, M. | |
| dc.date.accessioned | 2014-07-23T13:25:00Z | |
| dc.date.available | 2014-07-23T13:25:00Z | |
| dc.date.issued | 2002 | |
| dc.description.abstract | Vanadate solutions as ‘metavanadate’ (containing ortho and metavanadate species) and ‘decavanadate’ (containing manly decameric species) (5 mM; 1 mg/kg) were injected intraperitoneously in Halobatrachus didactylus (toadfish), in order to evaluate the contribution of decameric vanadate species to vanadium (V) intoxication on the cardiac tissue. Following short-term exposure (1 and 7 days), different changes on antioxidant enzyme activities—superoxide dismutase (SOD), catalase (CAT), selenium-glutathione peroxidase (Se-GPx), total glutathione peroxidase (GPx), lipid peroxidation and subcellular vanadium distribution were observed in mitochondrial and cytosolic fractions of heart ventricle toadfish. After 1 day of vanadium intoxication, SOD, CAT and Se-GPx activities were decreased up to 25%, by both vanadate solutions, except mitochondrial CAT activity that increased (123%) upon decavanadate administration. After 7 days of exposure, decavanadate versus metavanadate solutions promoted different effects mainly on cytosolic CAT activity (256% versus25%), mitochondrial CAT activity (210% versus110%) and total GPx activity (11% versus235%), whereas lipid peroxidation products were significantly increased (182%) upon 500mM decavanadate intoxication. Accumulation of vanadium in total (0.13760.011mg/g) and mitochondrial (0.02260.001mg/g) fractions was observed upon 7 days of metavanadate exposure, whereas for decavanadate, the concentration of vanadium increased in cytosolic (0.02060.005mg/g) and mitochondrial (0.02160.009mg/g) fractions. It is concluded that decameric vanadate species are responsible for a strong increase on lipid peroxidation and a decrease in cytosolic catalase activity thus contributing to oxidative stress responses upon vanadate intoxication, in the toadfish heart. | por |
| dc.identifier.issn | 0162-0134 | |
| dc.identifier.other | AUT: MAA01296 | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/4814 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Elsevier | por |
| dc.subject | Oxidative stress | por |
| dc.subject | Vanadate | por |
| dc.title | Oxidative stress in toadfish (Halobactrachus didactylus) cardiac muscle Acute exposure to vanadate oligomers | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 165 | por |
| oaire.citation.startPage | 159 | por |
| oaire.citation.title | Journal of Inorganic Biochemistry | por |
| oaire.citation.volume | 90 | por |
| person.familyName | Aureliano | |
| person.givenName | Manuel | |
| person.identifier | 584146 | |
| person.identifier.ciencia-id | AA14-3490-DC5E | |
| person.identifier.orcid | 0000-0003-4858-3201 | |
| person.identifier.rid | I-3283-2012 | |
| person.identifier.scopus-author-id | 6603412860 | |
| rcaap.rights | restrictedAccess | por |
| rcaap.type | article | por |
| relation.isAuthorOfPublication | bb413661-7edd-4b57-8338-33889cfd05db | |
| relation.isAuthorOfPublication.latestForDiscovery | bb413661-7edd-4b57-8338-33889cfd05db |