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Effect of essential oils on the release of TNF-α and CCL2 by LPS-Stimulated THP‑1 Cells

2020Journal article Open access
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dc.contributor.authorMiguel, Maria Graca
dc.contributor.authorSilva, Carina Isabel da
dc.contributor.authorFarah, Luana
dc.contributor.authorCastro Braga, Fernão
dc.contributor.authorFigueiredo, Ana Cristina
dc.date.accessioned2021-02-15T10:47:56Z
dc.date.available2021-02-15T10:47:56Z
dc.date.issued2020
dc.description.abstractPlants and their constituents have been used to treat diverse ailments since time immemorial. Many plants are used in diverse external and internal formulations (infusions, alcoholic extracts, essential oils (EOs), etc.) in the treatment of inflammation-associated diseases, such as those affecting the respiratory tract or causing gastrointestinal or joint problems, among others. To support the traditional uses of plant extracts, EOs have been assessed for their alleged anti-inflammatory properties. However, the effect of EOs on the release of cytokines and chemokines has been much less reported. Considering their traditional use and commercial relevance in Portugal and Angola, this study evaluated the effect of EOs on the in vitro inhibition of the cytokine tumor necrosis factor-α (TNF-α) and the chemokine (C-C motif) ligand 2 (CCL2) by lipopolysaccharide (LPS)-stimulated human acute monocytic leukemia cells (THP-1 cells). Twenty EOs extracted from eighteen species from seven families, namely from Amaranthaceae (Dysphania ambrosioides), Apiaceae (Foeniculum vulgare), Asteraceae (Brachylaena huillensis, Solidago virgaurea), Euphorbiaceae (Spirostachys africana), Lamiaceae (Lavandula luisieri, Mentha cervina, Origanum majorana, Satureja montana, Thymbra capitata, Thymus mastichina, Thymus vulgaris, Thymus zygis subsp. zygis), Myrtaceae (Eucalyptus globulus subsp. maidenii, Eucalyptus radiata, Eucalyptus viminalis) and Pinaceae (Pinus pinaster) were assayed for the release of CCL2 and TNF-α by LPS-stimulated THP-1 cells. B. huillensis, S. africana, S. montana, Th. mastichina and Th. vulgaris EOs showed toxicity to THP-1 cells, at the lowest concentration tested (10 μg/mL), using the tetrazolium dye assay. The most active EOs in reducing TNF-α release by LPS-stimulated THP-1 cells were those of T. capitata (51% inhibition at 20 μg/mL) and L. luisieri (15-23% inhibition at 30 μg/mL and 78-83% inhibition at 90 μg/mL). L. luisieri EO induced a concentration-dependent inhibition of CCL2 release by LPS‑stimulated THP-1 cells (23%, 54% and 82% inhibition at 10, 30 and 90 μg/mL, respectively). These EOs are potentially useful in the management of inflammatory diseases mediated by CCL2 and TNF‑α, such as atherosclerosis and arthritis.pt_PT
dc.description.sponsorshipFundação para a Ciência e Tecnologia (FCT/MCTES): MED , CESAM , FEDER, PT2020 PA / Compete 2020.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/plants10010050pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.1/15086
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation50017/2020pt_PT
dc.relationMediterranean Institute for Agriculture, Environment and Development
dc.relationCentre for Environmental and Marine Studies
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEssential oilspt_PT
dc.subjectInflammationpt_PT
dc.subjectCytokinept_PT
dc.subjectChemokinept_PT
dc.titleEffect of essential oils on the release of TNF-α and CCL2 by LPS-Stimulated THP‑1 Cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMediterranean Institute for Agriculture, Environment and Development
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F05183%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50017%2F2020/PT
oaire.citation.issue1pt_PT
oaire.citation.startPage50pt_PT
oaire.citation.titlePlantspt_PT
oaire.citation.volume10pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameMiguel
person.givenNameMaria
person.identifier.orcid0000-0002-1601-5501
person.identifier.scopus-author-id9941792100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationecda84a3-0649-4504-abb0-9c4c56ff862c
relation.isAuthorOfPublication.latestForDiscoveryecda84a3-0649-4504-abb0-9c4c56ff862c
relation.isProjectOfPublication823bd652-ee6c-4479-91c5-06095bc37e9c
relation.isProjectOfPublication8a6a1709-4374-4562-9853-faada5bcbc34
relation.isProjectOfPublication.latestForDiscovery823bd652-ee6c-4479-91c5-06095bc37e9c

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