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Quantitative assessment of the regenerative and mineralogenic performances of the zebrafish caudal fin

2016-12Journal article Open access
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dc.contributor.authorCardeira Da Silva, João
dc.contributor.authorGavaia, Paulo J.
dc.contributor.authorFernandez, Ignacio
dc.contributor.authorCengiz, Ibrahim Fatih
dc.contributor.authorMoreira-Silva, Joana
dc.contributor.authorOliveira, Joaquim Miguel
dc.contributor.authorReis, Rui L.
dc.contributor.authorCancela, Leonor
dc.contributor.authorLaizé, Vincent
dc.date.accessioned2017-04-07T15:55:33Z
dc.date.available2017-04-07T15:55:33Z
dc.date.issued2016-12
dc.description.abstractThe ability of zebrafish to fully regenerate its caudal fin has been explored to better understand the mechanisms underlying de novo bone formation and to develop screening methods towards the discovery of compounds with therapeutic potential. Quantifying caudal fin regeneration largely depends on successfully measuring new tissue formation through methods that require optimization and standardization. Here, we present an improved methodology to characterize and analyse overall caudal fin and bone regeneration in adult zebrafish. First, regenerated and mineralized areas are evaluated through broad, rapid and specific chronological and morphometric analysis in alizarin red stained fins. Then, following a more refined strategy, the intensity of the staining within a 2D longitudinal plane is determined through pixel intensity analysis, as an indicator of density or thickness/volume. The applicability of this methodology on live specimens, to reduce animal experimentation and provide a tool for in vivo tracking of the regenerative process, was successfully demonstrated. Finally, the methodology was validated on retinoic acid-and warfarin-treated specimens, and further confirmed by micro-computed tomography. Because it is easily implementable, accurate and does not require sophisticated equipment, the present methodology will certainly provide valuable technical standardization for research in tissue engineering, regenerative medicine and skeletal biology.
dc.description.sponsorshipPD/00117/2012
dc.identifier.doi10.1038/srep39191
dc.identifier.issn2045-2322
dc.identifier.otherAUT: LCA00739;
dc.identifier.urihttp://hdl.handle.net/10400.1/9158
dc.language.isoeng
dc.peerreviewedyes
dc.relationMECHANISMS OF DE NOVO BONE FORMATION IN A FISH MODEL OF FIN REGENERATION
dc.relationINTEGRATIVE ROLES OF VITAMIN K: NUTRITIONAL REQUIREMENTS FOR BETTER BONE DEVELOPMENT
dc.relationTissue engineering approaches for regeneration of human articulation
dc.relationMulti-functional Dendrimeric Macromolecular Systems for Application in Theranostics of Gastrointestinal Stromal Tumors (DendriGIST)
dc.relationISOLATION OF FISH BONE MORPHOGENETIC PROTEINS WITH OSTEOGENIC ACTIVITY FOR BONE REPAIR & REGENERATION
dc.relationAQUATOX - Assessment of mineralogenic toxicity of aquatic pollutants
dc.relationStrategic Project - LA 15 - 2011-2012
dc.relation.isbasedonWOS:000390229800001
dc.titleQuantitative assessment of the regenerative and mineralogenic performances of the zebrafish caudal fin
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMECHANISMS OF DE NOVO BONE FORMATION IN A FISH MODEL OF FIN REGENERATION
oaire.awardTitleINTEGRATIVE ROLES OF VITAMIN K: NUTRITIONAL REQUIREMENTS FOR BETTER BONE DEVELOPMENT
oaire.awardTitleTissue engineering approaches for regeneration of human articulation
oaire.awardTitleMulti-functional Dendrimeric Macromolecular Systems for Application in Theranostics of Gastrointestinal Stromal Tumors (DendriGIST)
oaire.awardTitleISOLATION OF FISH BONE MORPHOGENETIC PROTEINS WITH OSTEOGENIC ACTIVITY FOR BONE REPAIR & REGENERATION
oaire.awardTitleAQUATOX - Assessment of mineralogenic toxicity of aquatic pollutants
oaire.awardTitleStrategic Project - LA 15 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//PD%2FBD%2F52425%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F82049%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F99555%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00423%2F2012%2FCP0177%2FCT0004/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F70230%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR%2F112992%2F2009/PT
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oaire.citation.endPage39191
oaire.citation.startPage39191
oaire.citation.titleScientific Reports
oaire.citation.volume6
oaire.fundingStreamPOR_NORTE
oaire.fundingStreamInvestigador FCT
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
oaire.fundingStream6820 - DCRRNI ID
person.familyNameCardeira da Silva
person.familyNameGavaia
person.familyNameCancela
person.familyNameLaizé
person.givenNameJoão
person.givenNamePaulo
person.givenNameM. Leonor
person.givenNameVincent
person.identifier.ciencia-idDC15-86F7-3AAC
person.identifier.ciencia-idB619-FC16-D007
person.identifier.orcid0000-0003-3722-8810
person.identifier.orcid0000-0002-9582-1957
person.identifier.orcid0000-0003-3114-6662
person.identifier.orcid0000-0001-9565-9198
person.identifier.ridA-6470-2011
person.identifier.ridB-4463-2008
person.identifier.scopus-author-id55224020100
person.identifier.scopus-author-id6507104377
person.identifier.scopus-author-id6602982778
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
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