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Factors affecting in vitro adventitious shoot formation on internode explants of Citrus aurantium L. cv. Brazilian

2011Journal article Restricted access
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dc.contributor.authorMarques, N T.
dc.contributor.authorNolasco, Gustavo
dc.contributor.authorLeitão, J. M.
dc.date.accessioned2015-06-19T14:36:25Z
dc.date.available2015-06-19T14:36:25Z
dc.date.issued2011
dc.description.abstractThe in vitro formation of newly formed adventitious buds and shoots from internodal branch segments was studied on 12-month-old plants of Citrus aurantium L cv. Brazilian. The effects of 6-Benzyladenine (BA) and alpha-Naphthalene acetic acid (NAA) treatments were evaluated on adventitious bud and shoot regeneration. High rates of bud initiation and shoot development were obtained both with BA supplemented medium, in the range from 1 mg L(-1) to 3 mg L(-1), and with 0.1 mg L(-1) NAA supplemented medium. NAA concentrations above 1 mg L(-1) significantly reduced bud initiation and shoot elongation. The results obtained using different in vitro culture vessels such as Petri dishes, tubes and glass culture jars were compared. The highest adventitious bud induction was observed in Petri dishes for internodes cultured in 2 mg L(-1) BA supplemented medium, with 95% responsive explants forming 9.0 +/- 2.4 adventitious buds. The adventitious buds observed in Petri dishes reached a maximum height of 1 mm, with no further development, while some of the adventitious shoots cultured in tubes and glass culture jars grew over 1 cm in height. A shoot regeneration gradient of the internodes collected along the branch axis was noticed, with basal ones exhibiting higher regeneration frequency. (C) 2011 Elsevier B.V. All rights reserved.
dc.identifier.doihttps://dx.doi.org/10.1016/j.scienta.2011.03.018
dc.identifier.issn0304-4238
dc.identifier.otherAUT: NMA00640; GNO00324; JLE00446;
dc.identifier.urihttp://hdl.handle.net/10400.1/6495
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElesvier
dc.relation.isbasedonP-002-QGP
dc.titleFactors affecting in vitro adventitious shoot formation on internode explants of Citrus aurantium L. cv. Brazilian
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage182
oaire.citation.startPage176
oaire.citation.titleScientia Horticulturae
oaire.citation.volume129
person.familyNameTomás Marques
person.familyNameNolasco
person.familyNameLeitão
person.givenNameNatália
person.givenNameGustavo
person.givenNameJosé
person.identifier258398
person.identifier.ciencia-id1510-CD2F-81CE
person.identifier.ciencia-id7A17-3E0A-73AD
person.identifier.orcid0000-0002-0222-621X
person.identifier.orcid0000-0002-7025-6023
person.identifier.orcid0000-0003-4278-0415
person.identifier.ridAAH-3958-2020
person.identifier.scopus-author-id6602625528
person.identifier.scopus-author-id7005119796
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication4b1751aa-958b-4d68-ae97-ea628673ba00
relation.isAuthorOfPublication87a8035f-bc15-44dd-b8c3-4288c5660fff
relation.isAuthorOfPublication0f990d1c-2913-4f8a-8fb6-f8b02721247f
relation.isAuthorOfPublication.latestForDiscovery4b1751aa-958b-4d68-ae97-ea628673ba00

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