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Revisiting IgG antibody reactivity to epstein-barr virus in myalgic encephalomyelitis/chronic fatigue syndrome and Its potential application to disease diagnosis

2022Journal article Open Access
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dc.contributor.authorSepúlveda, Nuno
dc.contributor.authorMalato, João
dc.contributor.authorSotzny, Franziska
dc.contributor.authorGrabowska, Anna D.
dc.contributor.authorFonseca, André
dc.contributor.authorCordeiro, Clara
dc.contributor.authorGraça, Luís
dc.contributor.authorBiecek, Przemyslaw
dc.contributor.authorBehrends, Uta
dc.contributor.authorMautner, Josef
dc.contributor.authorWestermeier, Francisco
dc.contributor.authorLacerda, Eliana M.
dc.contributor.authorScheibenbogen, Carmen
dc.date.accessioned2022-12-16T13:01:39Z
dc.date.available2022-12-16T13:01:39Z
dc.date.issued2022
dc.description.abstractInfections by the Epstein-Barr virus (EBV) are often at the disease onset of patients suffering from Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS). However, serological analyses of these infections remain inconclusive when comparing patients with healthy controls (HCs). In particular, it is unclear if certain EBV-derived antigens eliciting antibody responses have a biomarker potential for disease diagnosis. With this purpose, we re-analyzed a previously published microarray data on the IgG antibody responses against 3,054 EBV-related antigens in 92 patients with ME/CFS and 50 HCs. This re-analysis consisted of constructing different regression models for binary outcomes with the ability to classify patients and HCs. In these models, we tested for a possible interaction of different antibodies with age and gender. When analyzing the whole data set, there were no antibody responses that could distinguish patients from healthy controls. A similar finding was obtained when comparing patients with non-infectious or unknown disease trigger with healthy controls. However, when data analysis was restricted to the comparison between HCs and patients with a putative infection at their disease onset, we could identify stronger antibody responses against two candidate antigens (EBNA4_0529 and EBNA6_0070). Using antibody responses to these two antigens together with age and gender, the final classification model had an estimated sensitivity and specificity of 0.833 and 0.720, respectively. This reliable case-control discrimination suggested the use of the antibody levels related to these candidate viral epitopes as biomarkers for disease diagnosis in this subgroup of patients. To confirm this finding, a follow-up study will be conducted in a separate cohort of patients.pt_PT
dc.description.sponsorshipPPN/ULM/2020/1/00069/U/00001; SCIO Charity Number SC036942; (ref. NIH 2R01AI103629) ; Grant PF8947_ME Association
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3389/fmed.2022.921101pt_PT
dc.identifier.eissn2296-858X
dc.identifier.urihttp://hdl.handle.net/10400.1/18649
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontiers Media SApt_PT
dc.relationCentre of Statistics and its Applications
dc.relationRegulatory T cells and their role on the aetiology of Myalgic encephalomyelitis/chronic fatigue syndrome
dc.relationAnalysis of high-throughput antibody data for better understanding of immunogenetics and epidemiology of malaria
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEpstein-Barr viruspt_PT
dc.subjectMyalgic encephalomyelitispt_PT
dc.subjectChronic fatigue syndromept_PT
dc.subjectAntigen mimicrypt_PT
dc.subjectBiomarker discoverypt_PT
dc.subjectPatient stratificationpt_PT
dc.titleRevisiting IgG antibody reactivity to epstein-barr virus in myalgic encephalomyelitis/chronic fatigue syndrome and Its potential application to disease diagnosispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre of Statistics and its Applications
oaire.awardTitleRegulatory T cells and their role on the aetiology of Myalgic encephalomyelitis/chronic fatigue syndrome
oaire.awardTitleAnalysis of high-throughput antibody data for better understanding of immunogenetics and epidemiology of malaria
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F149758%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F147629%2F2019/PT
oaire.citation.titleFrontiers in Medicinept_PT
oaire.citation.volume9pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
person.familyNameFonseca
person.familyNameHenrique Cordeiro
person.givenNameAndré Filipe Afonso de Sousa
person.givenNameClara Maria
person.identifier.ciencia-id0B1D-8695-6ADB
person.identifier.ciencia-idC71E-21A1-E882
person.identifier.orcid0000-0002-1026-6078
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication7e0bbaff-c584-461a-84e2-54f2a6c15876
relation.isAuthorOfPublication.latestForDiscoveryc242bab5-7d06-4b31-9fae-98447f5e2b96
relation.isProjectOfPublication100e6d17-2f76-4282-b864-d0f887b34243
relation.isProjectOfPublication6a4bb66a-47b3-4c7a-8331-682d5c0393ed
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