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Evidences for a new role of miR-214 in chondrogenesis

2018-02Journal article Open access
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dc.contributor.authorRoberto, Vania Palma
dc.contributor.authorGavaia, Paulo
dc.contributor.authorNunes, Maria Joao
dc.contributor.authorRodrigues, Elsa
dc.contributor.authorM. Leonor Cancela
dc.contributor.authorTiago, Daniel
dc.date.accessioned2018-12-07T14:53:03Z
dc.date.available2018-12-07T14:53:03Z
dc.date.issued2018-02
dc.description.abstractmiR-214 is known to play a role in mammalian skeletal development through inhibition of osteogenesis and stimulation of osteoclastogenesis, but data regarding other vertebrates, as well as a possible role in chondrogenesis, remain unknown. Here, we show that miR-214 expression is detected in bone and cartilage of zebrafish skeleton, and is downregulated during murine ATDC5 chondrocyte differentiation. Additionally, we observed a conservation of the transcriptional regulation of miR-214 primary transcript Dnm3os in vertebrates, being regulated by Ets1 in ATDC5 chondrogenic cells. Moreover, overexpression of miR-214 in vitro and in vivo mitigated chondrocyte differentiation probably by targeting activating transcription factor 4 (Atf4). Indeed, miR-214 overexpression in vivo hampered cranial cartilage formation of zebrafish and coincided with downregulation of atf4 and of the key chondrogenic players sox9 and col2a1. We show that miR-214 overexpression exerts a negative role in chondrogenesis by impacting on chondrocyte differentiation possibly through conserved mechanisms.
dc.description.sponsorshipCalouste Gulbenkian Foundation (program "Na Fronteira das Ciencias da Vida"); FCT [UID/Multi/04326/2013, PEst-C/MAR/LA0015/2011, SFRH/BD/38607/2007, SFRH/BPD/45034/2008, SFRH/BPD/111289/2015]; European Commission (ERDF-COMPETE) [PEst-C/MAR/LA0015/2011]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1038/s41598-018-21735-w
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10400.1/11329
dc.language.isoeng
dc.peerreviewedyes
dc.publisherNature Publishing Group
dc.relationZEBRAFISH AS A TRANSGENIC IN VIVO MODEL TO ANALYSE BONE-RELATED GENE FUNCTION AND REGULATION OF EXPRESSION
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectZebrafish Danio-Rerio
dc.subjectMatrix gla protein
dc.subjectCranial neural crest
dc.subjectSkeletal development
dc.subjectBone-formation
dc.subjectOsteoblast differentiation
dc.subjectGene-expression
dc.subjectOsteogenic Differentiation
dc.subjectGilthead seabream
dc.subjectCell lineage
dc.titleEvidences for a new role of miR-214 in chondrogenesis
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleZEBRAFISH AS A TRANSGENIC IN VIVO MODEL TO ANALYSE BONE-RELATED GENE FUNCTION AND REGULATION OF EXPRESSION
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F38607%2F2007/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F45034%2F2008/PT
oaire.citation.startPage3704
oaire.citation.titleScientific Reports
oaire.citation.volume8
oaire.fundingStream5876
oaire.fundingStreamSFRH
person.familyNameRoberto
person.familyNameGavaia
person.familyNameNunes
person.familyNameRodrigues
person.familyNameCancela
person.familyNameTiago
person.givenNameVĂąnia
person.givenNamePaulo
person.givenNameMaria JoĂŁo
person.givenNameElsa
person.givenNameM. Leonor
person.givenNameDaniel
person.identifier1230353
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person.identifier.ciencia-idCE1D-5FF6-DA93
person.identifier.orcid0000-0002-8134-4045
person.identifier.orcid0000-0002-9582-1957
person.identifier.orcid0000-0002-4922-633X
person.identifier.orcid0000-0003-1669-365X
person.identifier.orcid0000-0003-3114-6662
person.identifier.orcid0000-0001-8418-6292
person.identifier.ridA-6470-2011
person.identifier.ridF-6766-2015
person.identifier.ridJ-3100-2012
person.identifier.scopus-author-id27467657800
person.identifier.scopus-author-id6507104377
person.identifier.scopus-author-id24780762700
person.identifier.scopus-author-id57197191479
person.identifier.scopus-author-id14422711000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a CiĂȘncia e a Tecnologia
project.funder.nameFundação para a CiĂȘncia e a Tecnologia
project.funder.nameFundação para a CiĂȘncia e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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