Repository logo
 
Publication

Olfactory sensitivity to catecholamines and their metabolites in the goldfish

2003Journal article Restricted access
Simple item page
dc.contributor.authorHubbard, Peter
dc.contributor.authorBarata, E. N.
dc.contributor.authorCanario, Adelino V. M.
dc.date.accessioned2014-05-14T09:48:44Z
dc.date.available2014-05-14T09:48:44Z
dc.date.issued2003
dc.date.updated2014-05-09T09:40:16Z
dc.description.abstractThe current study assessed the olfactory sensitivity of the goldfish (Carassius auratus L.) to the catecholamines, their immediate precursors and metabolites by use of the electro-olfactogram (EOG). The olfactory system of the goldfish was found to be sensitive to both adrenaline and dopamine with thresholds of detection of 10–7.8 and 10–7.9 M respectively, but less so to noradrenaline (threshold of detection 10–6.3 M). The 3-O-methoxy metabolites (metadrenaline, normetadrenaline and 3-O-methoxytyramine) evoked larger amplitude EOGs than the non-metabolized form with lower thresholds of detection. However, the olfactory system was less sensitive to the amino acid precursors L-tyrosine and L-DOPA, and markedly less so to the α-deaminated metabolites (3,4-dihydroxyphenyl glycol, 3,4-dihydroxy mandelic acid and dihydroxyphenyacetic acid). Sensitivity to metabolites, both α-deaminated and 3-O-methoxylated, was similar to the α-deaminated forms. Crossadaptation studies suggested that, while there is some degree of commonality of the receptor mechanisms with L-tyrosine and L-serine, a proportion of the response to the catecholamines is due to distinct receptor subtypes. Similarly, the 3-O-methoxy metabolites also had (a) separate receptor mechanism(s), although, again, there was overlap with the adrenaline/dopamine receptor site(s). Presence of the α-adrenoreceptor antagonist prazosin or the peripheral DA2 dopamine receptor antagonist domperidone caused partial attenuation of the EOG responses to adrenaline and dopamine, but had much less effect on the responses to their 3-O-methoxy metabolites. The β-adrenoreceptor antagonist sotalol had no such effect. This suggests that the olfactory catecholamine receptors are structurally and functionally distinct from systemic adreno- and dopamine receptors. The current study raises the possibility that release of catecholamines or their 3-O-methoxy metabolites to the water may play a role in chemical communication.por
dc.identifier.citationHubbard, P. C.; Barata, E. N.; Canario, A. V. M. Olfactory sensitivity to catecholamines and their metabolites in the goldfish, Chemical Senses, 28, 3, 207-218, 2003.por
dc.identifier.issn0379-864X
dc.identifier.otherAUT: ACA00258;
dc.identifier.urihttp://hdl.handle.net/10400.1/3923
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherOxford University Presspor
dc.subjectMetabolitespor
dc.subjectTeleostpor
dc.subjectAdrenalinepor
dc.subjectDopaminepor
dc.subjectElectro-olfactogram (EOG)por
dc.titleOlfactory sensitivity to catecholamines and their metabolites in the goldfishpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage218por
oaire.citation.issue3por
oaire.citation.startPage207por
oaire.citation.titleChemical Sensespor
oaire.citation.volume28por
person.familyNameHubbard
person.familyNameBarata
person.familyNameCanario
person.givenNamePeter
person.givenNameEduardo Nuno
person.givenNameAdelino
person.identifier143624
person.identifier.ciencia-id951F-BA4B-A21F
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0002-3007-4647
person.identifier.orcid0000-0001-9714-5967
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridB-2823-2008
person.identifier.ridB-2902-2010
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id7005928467
person.identifier.scopus-author-id6602073256
person.identifier.scopus-author-id56568523700
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationf599b3ac-806b-4f65-9eb6-f70e68bcb142
relation.isAuthorOfPublicationa2bb6982-e4c8-4ea3-b160-bc3464cd960f
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscovery5f6e51ee-9113-469e-8b9e-f30f2d452521

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Olfactory Sensitivity to Catecholamines and their Metabolites in the Goldfish .pdf
Size:
326.07 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.61 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)