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Micropropagation of the Mediterranean tree Ceratonia siliqua

dc.contributor.authorRomano, Anabela
dc.contributor.authorBarros, S.
dc.contributor.authorMartins-Loução, M. A.
dc.date.accessioned2015-06-18T10:12:35Z
dc.date.available2015-06-18T10:12:35Z
dc.date.issued2002
dc.description.abstractAn in vitro propagation protocol based on axillary bud proliferation has been developed for mature female trees of Ceratonia siliqua L. 'Galhosa' and 'Mulata'. Browning and contaminants were the major obstacles for culture establishment. Shoot culture initiation was greatly influenced by explanting season, with the highest survival percentage observed in spring. The cultivar, cytokinin type and concentration were the most important factors affecting shoot multiplication. The best multiple-shoot response was obtained with 'Mulata' on Murashige and Skoog medium supplemented with 4.44 muM 6-benzyladenine or 4.56 muM zeatin. Rooting was achieved on growth-regulator-free medium after basal dipping of shoots in indole-3-butyric acid (4.9 mM). Plantlets were successfully acclimatized (80-85%) under high relative humidity and then moved to the glasshouse. A field trial was established to follow their agronomic behaviour.
dc.identifier.doihttps://dx.doi.org/10.1023/A:1012912504288
dc.identifier.issn0167-6857
dc.identifier.otherAUT: ARO01015;
dc.identifier.urihttp://hdl.handle.net/10400.1/6411
dc.language.isoeng
dc.peerreviewedyes
dc.publisherKluwer Academic Publishers
dc.relation.isbasedonP-000-RSK
dc.titleMicropropagation of the Mediterranean tree Ceratonia siliqua
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage41
oaire.citation.startPage35
oaire.citation.titlePlant Cell, Tissue and Organ Culture
oaire.citation.volume68
person.familyNameRomano
person.givenNameAnabela
person.identifier.ciencia-id8A18-7E9A-3B0E
person.identifier.orcid0000-0002-7204-7428
person.identifier.scopus-author-id56249997600
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublicatione3343b60-17d3-4f5e-a2bf-06f26da69206
relation.isAuthorOfPublication.latestForDiscoverye3343b60-17d3-4f5e-a2bf-06f26da69206

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