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Molecular detection of Leishmania DNA and identification of blood meals in wild caught phlebotomine sand flies (Diptera: Psychodidae) from southern Portugal

2015Journal article Open Access
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dc.contributor.authorMaia, Carla
dc.contributor.authorParreira, Ricardo
dc.contributor.authorCristovao, Jose Manuel
dc.contributor.authorFreitas, Ferdinando Bernardino
dc.contributor.authorAfonso, Maria Odete
dc.contributor.authorCampino, Lenea
dc.date.accessioned2018-12-07T14:57:57Z
dc.date.available2018-12-07T14:57:57Z
dc.date.issued2015
dc.description.abstractBackground: Zoonotic visceral leishmaniasis caused by Leishmania infantum which is transmitted by phlebotomine sand flies (Diptera, Psychodidae) is endemic in the Mediterranean basin. The main objectives of this study were to (i) detect Leishmania DNA and (ii) identify blood meal sources in wild caught female sand flies in the zoonotic leishmaniasis region of Algarve, Portugal/Southwestern Europe. Methods: Phlebotomine sand flies were collected using CDC miniature light traps and sticky papers. Sand flies were identified morphologically and tested for Leishmania sp. by PCR using ITS-1 as the target sequence. The source of blood meal of the engorged females was determined using the cyt-b sequence. Results: Out of the 4,971 (2,584 males and 2,387 females) collected sand flies, Leishmania DNA was detected by PCR in three females (0.13%), specifically in two specimens identified on the basis of morphological features as Sergentomyia minuta and one as Phlebotomus perniciosus. Haematic preferences, as defined by the analysis of cyt-b DNA amplified from the blood-meals detected in the engorged female specimens, showed that P. perniciosus fed on a wide range of domestic animals while human and lizard DNA was detected in engorged S. minuta. Conclusions: The anthropophilic behavior of S. minuta together with the detection of Leishmania DNA highlights the need to determine the role played by this species in the transmission of Leishmania parasites to humans. In addition, on-going surveillance on Leishmania vectors is crucial as the increased migration and travelling flow elevate the risk of introduction and spread of infections by Leishmania species which are non-endemic.
dc.description.sponsorshipEU [FP7-261504]; Fundacao para a Ciencia e a Tecnologia, Ministerio da Educacao e Ciencia, Portugal [SFRH/BPD/44082/2008]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1186/s13071-015-0787-4
dc.identifier.issn1756-3305
dc.identifier.urihttp://hdl.handle.net/10400.1/11781
dc.language.isoeng
dc.peerreviewedyes
dc.publisherBMC
dc.relationDEVELOPMENT OF AN EXPERIMENTAL MODEL OF LEISHMANIA INFANTUM FOR THE EVALUATION OF VACCINE CANDIDATES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCutaneous Leishmaniasis
dc.subjectCanine Leishmaniasis
dc.subjectSergentomyia-Minuta
dc.subjectFeeding preferences
dc.subjectNatural infection
dc.subjectAlgarve region
dc.subject1St detection
dc.subjectFocus
dc.subjectPerniciosus
dc.subjectVector
dc.titleMolecular detection of Leishmania DNA and identification of blood meals in wild caught phlebotomine sand flies (Diptera: Psychodidae) from southern Portugal
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDEVELOPMENT OF AN EXPERIMENTAL MODEL OF LEISHMANIA INFANTUM FOR THE EVALUATION OF VACCINE CANDIDATES
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F44082%2F2008/PT
oaire.citation.endPage10
oaire.citation.issue173
oaire.citation.startPage1
oaire.citation.titleParasites and Vectors
oaire.citation.volume8
person.familyNameCampino
person.givenNameLenea
person.identifier.ciencia-id2A16-58CC-48FE
person.identifier.orcid0000-0003-4512-9660
person.identifier.scopus-author-id6603808132
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication408b2ada-2070-4192-8710-aebcd7eba09a
relation.isAuthorOfPublication.latestForDiscovery408b2ada-2070-4192-8710-aebcd7eba09a
relation.isProjectOfPublication0efe32c2-1236-42f8-9e7f-5ee4ed9023ff
relation.isProjectOfPublication.latestForDiscovery0efe32c2-1236-42f8-9e7f-5ee4ed9023ff

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