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Nucleic acid derived indices or instantaneous growth rate as tools to determine different nutricional condition in cuttlefish (Sepia officinalis, linnaeus 1758) hatchlings

dc.contributor.authorSykes, António V.
dc.contributor.authorDomingues, Pedro M.
dc.contributor.authorAndrade, José Pedro
dc.date.accessioned2018-11-12T19:54:53Z
dc.date.available2018-11-12T19:54:53Z
dc.date.issued2004
dc.description.abstractTwo groups of 100 cuttlefish hatchlings each were used to determine the duration of the yolk reserves, during which growth can be obtained with no food supply. One group was fed live grass shrimp ad libitum from the 3rd day of life onwards, while the other was starved during the whole experiment. The experiment lasted 7 days, because this was the 1st day where a negative growth rate was obtained. Nucleic acid derived indices and instantaneous growth rates (IGR) were used as a way to describe their condition, when all the yolk reserves would be exhausted and to determine the most accurate tool to express growth and condition. RNA/DNA ratios described clearly the differences (P < 0.05) between fed and starved hatchlings cultured at 23 ± 2°C, especially from day 4 onwards. Correlation between IGR and RNA/DNA ratios was only attained for the starved population (R 0.90; P 0.0374). DNA concentration was identical (P > 0.05) between groups and did not displayed a clear pattern with increasing age. Correlation between IGR and DNA was only attained for the fed population (R −0.90; P 0.0374). RNA concentration values were different from day 5 onwards. Any of the nucleic acid derived indexes were not accurate enough to express growth and condition, so IGR seems to be the most accurate and inexpensive way to describe hatchlings growth and condition in controlled conditions.pt_PT
dc.description.sponsorship2/2.1/MAR/1707/95
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.issn0730-8000
dc.identifier.otherAUT: JAN00399;
dc.identifier.urihttp://hdl.handle.net/10400.1/10933
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNational Shellfisheries Associationpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCondition indicespt_PT
dc.subjectCuttlefishpt_PT
dc.subjectDNA/RNA ratiospt_PT
dc.subjectGrowth ratespt_PT
dc.subjectHatchlingspt_PT
dc.subjectAccuracy methodologiespt_PT
dc.subjectSepiapt_PT
dc.titleNucleic acid derived indices or instantaneous growth rate as tools to determine different nutricional condition in cuttlefish (Sepia officinalis, linnaeus 1758) hatchlingspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage591pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage585pt_PT
oaire.citation.titleJournal of Shellfish Researchpt_PT
oaire.citation.volume23pt_PT
person.familyNameSykes
person.familyNameAndrade
person.givenNameAntónio
person.givenNameJose
person.identifier107454
person.identifier.ciencia-id7510-6641-5A42
person.identifier.ciencia-idD514-1A21-F020
person.identifier.orcid0000-0002-5207-0612
person.identifier.orcid0000-0002-5482-9776
person.identifier.ridC-3609-2012
person.identifier.scopus-author-id7102626426
person.identifier.scopus-author-id7201722097
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb9d55f95-5de8-40cf-82a3-96ec98c91a55
relation.isAuthorOfPublication8f1ffbdc-85aa-4342-8bd8-61eb6d6be74e
relation.isAuthorOfPublication.latestForDiscovery8f1ffbdc-85aa-4342-8bd8-61eb6d6be74e

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