Repository logo
 
Publication

Vitamin A affects flatfish development in a thyroid hormone signaling and metamorphic stage dependent manner

2017-06Journal article Open access
Simple item page
dc.contributor.authorFernandez, Ignacio
dc.contributor.authorOrtiz-Delgado, Juan B.
dc.contributor.authorDarias, Maria J.
dc.contributor.authorHontoria, Francisco
dc.contributor.authorAndree, Karl B.
dc.contributor.authorManchado, Manuel
dc.contributor.authorSarasquete, Carmen
dc.contributor.authorGisbert, Enric
dc.date.accessioned2018-12-07T14:53:27Z
dc.date.available2018-12-07T14:53:27Z
dc.date.issued2017-06
dc.description.abstractVitamin A (VA) and retinoid derivatives are known morphogens controlling vertebrate development. Despite the research effort conducted during the last decade, the precise mechanism of how VA induces post-natal bone changes, and particularly those operating through crosstalk with the thyroid hormones (THs) remain to be fully understood. Since effects and mechanisms seem to be dose and time-dependent, flatfish are an interesting study model as they undergo a characteristic process of metamorphosis driven by THs that can be followed by external appearance. Here, we studied the effects of VA imbalance that might determine Senegalese sole (Solea senegalensis) skeletogenetic phenotype through development of thyroid follicles, THs homeostasis and signaling when a dietary VA excess was specifically provided during pre-, pro-or post-metamorphic stages using enriched rotifers and Artemia as carriers. The increased VA content in enriched live prey was associated to a higher VA content in fish at all developmental stages. Dietary VA content clearly affected thyroid follicle development, T3 and T4 immunoreactive staining, skeletogenesis and mineralization in a dose and time-dependent fashion. Gene expression analysis showed that VA levels modified the mRNA abundance of VA- and TH-specific nuclear receptors at specific developmental stages. Present results provide new and key knowledge to better understand how VA and TH pathways interact at tissue, cellular and nuclear level at different developmental periods in Senegalese sole, unveiling how dietary modulation might determine juvenile phenotype and physiology.
dc.description.sponsorshipMinistry of Education and Culture (MEC) of the Spanish Government [AGL2005-02478]; [SFRH/BPD/82049/2011]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.3389/fphys.2017.00458
dc.identifier.issn1664-042X
dc.identifier.urihttp://hdl.handle.net/10400.1/11520
dc.language.isoeng
dc.peerreviewedyes
dc.publisherFrontiers Media Sa
dc.relationINTEGRATIVE ROLES OF VITAMIN K: NUTRITIONAL REQUIREMENTS FOR BETTER BONE DEVELOPMENT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectSole Solea-Senegalensis
dc.subjectLarval Japanese Flounder
dc.subjectRetinoic acid receptors
dc.subjectLatris-Lineata Larvae
dc.subjectUnion-of-Pharmacology
dc.subjectEuropean Fish Larvae
dc.subjectReal-time Pcr
dc.subjectGene-expression
dc.subjectEnriched artemia
dc.subjectSparus-Aurata
dc.titleVitamin A affects flatfish development in a thyroid hormone signaling and metamorphic stage dependent manner
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleINTEGRATIVE ROLES OF VITAMIN K: NUTRITIONAL REQUIREMENTS FOR BETTER BONE DEVELOPMENT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F82049%2F2011/PT
oaire.citation.startPage458
oaire.citation.titleFrontiers in Physiology
oaire.citation.volume8
person.familyNameFernández Monzón
person.familyNameGisbert
person.givenNameIgnacio Valentín
person.givenNameEnric
person.identifier117775
person.identifier606918
person.identifier.ciencia-id111D-018F-70FE
person.identifier.orcid0000-0002-8088-7902
person.identifier.orcid0000-0002-7457-8468
person.identifier.ridB-1527-2017
person.identifier.ridB-3654-2011
person.identifier.scopus-author-id24484817300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublicatione27390ef-b969-4998-a13b-92a5164a151a
relation.isAuthorOfPublicationd45a0129-65f8-469d-a8d9-74c1d8c40284
relation.isAuthorOfPublication.latestForDiscoverye27390ef-b969-4998-a13b-92a5164a151a
relation.isProjectOfPublicationc92313d2-71c0-4b43-9593-b689bf4396a3
relation.isProjectOfPublication.latestForDiscoveryc92313d2-71c0-4b43-9593-b689bf4396a3

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
handle11520.pdf
Size:
4.04 MB
Format:
Adobe Portable Document Format
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)