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17a,20ß,21-trihydroxy-4-pregnen-3-one: the probable maturation-inducing steroid of the lusitanian toadfish

2002Journal article Restricted access
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dc.contributor.authorModesto, Teresa
dc.contributor.authorCanario, Adelino V. M.
dc.date.accessioned2014-05-14T12:46:36Z
dc.date.available2014-05-14T12:46:36Z
dc.date.issued2002
dc.date.updated2014-05-09T09:51:09Z
dc.description.abstract17,20 ,21-Trihydroxy-4-pregnen-3-one (17,20 ,21-P) was identified as the major metabolite of incubations of Lusitanian toadfish Halobatrachus didactylus ovarian follicles with [3H]-17hydroxyprogesterone. The potency of several steroids in inducing germinal vesicle breakdown of follicle-enclosed oocytes of Lusitanian toadfish was systematically examined by using an in vitro germinal vesicle breakdown (GVBD) bioassay. 17,20 -Dihydroxy-4-pregnen-3-one (17,20 -P) and 17,20 ,21-P, two confirmed maturation-inducing steroids (MIS) in teleosts, were the most potent in inducing GVBD with ED50s ranging between 9 and 271 nM. Structure-activity relationships followed similar patterns to what has been observed in similar bioassays, i.e. a vital requirement for 17- and 20 -hydroxyl groups in C21 steroids and a reduction in activity of 14 and 5–6%, respectively, for 5-pregnene and 5 -pregnanes compared to 4-pregnenes. Corticosteroids, testosterone and 17 -oestradiol were ineffective. Folliculated oocytes stimulated by pituitary homogenate produced 17,20 ,21-P from endogenous substrates in amounts one order of magnitude higher than 17,20 -P. These results strongly support the hypothesis that 17,20 ,21-P is the likely MIS in this species.por
dc.identifier.citationModesto, T; Adelino V. M. Canário. 17a,20ß,21-Trihydroxy-4-pregnen-3-one: the probable maturation-inducing steroid of the Lusitanian toadfish, Journal of Fish Biology, 60, 3, 637-648, 2002.por
dc.identifier.doihttp://dx.doi.org/10.1006/jfbi.2002.1881
dc.identifier.issn0022-1112
dc.identifier.otherAUT: TMO00411; ACA00258;
dc.identifier.urihttp://hdl.handle.net/10400.1/3932
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherThe Fisheries Society of the British Islespor
dc.subjectOocyte maturationpor
dc.subjectHalobatrachus didactyluspor
dc.subjectLusitanian toadfishpor
dc.subject17,20 ,21-trihydroxy-4-pregnen-3-onepor
dc.subjectMaturation-inducing hormonepor
dc.title17a,20ß,21-trihydroxy-4-pregnen-3-one: the probable maturation-inducing steroid of the lusitanian toadfishpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage648por
oaire.citation.issue3por
oaire.citation.startPage637por
oaire.citation.titleJournal of Fish Biologypor
oaire.citation.volume60por
person.familyNameModesto
person.familyNameCanario
person.givenNameTeresa
person.givenNameAdelino
person.identifier143624
person.identifier.ciencia-idBD1C-7610-B888
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0002-2691-4906
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id6603372075
person.identifier.scopus-author-id56568523700
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationfc9019ec-88bd-4fb1-a4b8-dd5eed1fd284
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscoveryfc9019ec-88bd-4fb1-a4b8-dd5eed1fd284

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