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Prokaryotic community structure and auxin biosynthesis in early developmental stages of farmed atlantic nori (Porphyra spp.)

2026-01-21Artigo científico Acesso aberto
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datacite.subject.sdg14:Proteger a Vida Marinha
datacite.subject.sdg02:Erradicar a Fome
datacite.subject.sdg09:Indústria, Inovação e Infraestruturas
dc.contributor.authorCortez, Francisco
dc.contributor.authorNanetti, Enrico
dc.contributor.authorChaves, Guilherme
dc.contributor.authorPereira, André C.
dc.contributor.authorCaria Mendes, Madalena
dc.contributor.authorOliveira, Inês
dc.contributor.authorLeuzzi, Daniela
dc.contributor.authorAbreu, Helena
dc.contributor.authorMartins, Margarida
dc.contributor.authorLeite, Ricardo B.
dc.contributor.authorKeller-Costa, Tina
dc.contributor.authorCosta, Rodrigo
dc.date.accessioned2026-03-05T10:32:48Z
dc.date.available2026-03-05T10:32:48Z
dc.date.issued2026-01-21
dc.description.abstractIntroduction: Algal-microbiome interactions are considered pivotal for host health and development. Current understanding of the diversity and function of algal-associated microorganisms in aquaculture settings remains limited, preventing the development of microbiome-based solutions for sustainable algal growth.Methods: We employed cultivation-dependent and -independent approaches to determine the structure of bacterial communities associated with farmed Atlantic Nori (Porphyra dioica and Porphyra umbilicalis) at early developmental stages. 16S rRNA gene amplicon sequencing and cultivation of bacterial symbionts were performed for algal and culturing water samples harvested from indoor photobioreactors at stages S1 (conchocelis cultures growing vegetatively), S2 (conchosporangia), and S3 (young blades).Results: The phyla Pseudomonadota (Alpha- and Gammaproteobacteria classes) and Bacteroidota were dominant in algal samples, followed by Planctomycetota, Actinobacteriota, and Verrucomicrobiota. At the phylotype level, these communities were highly structured throughout the host’s life cycle. Uncultivated lineages Sva0996 (Actinomycetota), OM190 (Planctomycetota), Pir4 (Planctomycetota), and the genera Blastopirellula, Algoriphagus, Hyphomonas, and Marinobacter, among others, were enriched in algal samples and presented significantly different abundances across developmental stages. In some cases (e.g., genera Aquimarina, Sulfitobacter, Maribacter, and Nonlabens), those changes were also observed in culturing water. Moreover, the genera Ensifer (Rhizobiaceae), Paraglaciecola (Alteromonadaceae), and the uncultivated lineages DEV007 (Verrucomicrobiota) and Pir4 (Planctomycetota) were consistently present in P. dioica and P. umbilicalis samples at multiple developmental stages. Several Porphyra-associated bacterial genera and putative novel species, mostly belonging to the families Roseobacteraceae, Flavobacteriaceae, and Alteromonadaceae were identified via cultivation. Many cultured members of the Porphyra microbiome produced the growth-promoting hormone auxin, particularly those belonging to the genera Alteromonas, Marinobacter, Sulfitobacter, Leucothrix, and Roseovarius.Discussion: This study unveils complex, phylogenetically distinct, and temporally structured bacterial communities possessing algal morphogenesis-inducing capacities during early developmental stages of Porphyra spp., highlighting the potential of microbiome-based interventions for sustainable growth of marine algae in aquaculture.eng
dc.description.sponsorshipCEECIND/00788/2017
dc.identifier.doi10.3389/fmicb.2025.1750184
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/10400.1/28334
dc.language.isoeng
dc.peerreviewedyes
dc.publisherFrontiers Media SA
dc.relationInstitute for Bioengineering and Biosciences
dc.relationInstitute for Bioengineering and Biosciences
dc.relationInstitute for Health and Bioeconomy
dc.relation.ispartofFrontiers in Microbiology
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject16S rRNA gene sequencing
dc.subjectAlgal morphogenesis
dc.subjectAlgal-microbiome interactions
dc.subjectAquaculture
dc.subjectAtlantic nori cultivation
dc.subjectMacroalgae
dc.titleProkaryotic community structure and auxin biosynthesis in early developmental stages of farmed atlantic nori (Porphyra spp.)eng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUIDB/04565/2020
oaire.awardNumberUIDP/04565/2020
oaire.awardNumberLA/P/0140/2020
oaire.awardTitleInstitute for Bioengineering and Biosciences
oaire.awardTitleInstitute for Bioengineering and Biosciences
oaire.awardTitleInstitute for Health and Bioeconomy
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04565%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04565%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0140%2F2020/PT
oaire.citation.titleFrontiers in Microbiology
oaire.citation.volume16
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameCaria Mendes
person.givenNameMadalena
person.identifier.ciencia-id9C12-FB3A-81F7
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isAuthorOfPublication2eab4c0e-9099-454d-8043-656a7710a381
relation.isAuthorOfPublication.latestForDiscovery2eab4c0e-9099-454d-8043-656a7710a381
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