Repository logo
 
Publication

The calcitonin-like system is an ancient regulatory system of biomineralization

2020Journal article Open access
Simple item page
dc.contributor.authorCardoso, João CR
dc.contributor.authorFélix, Rute C.
dc.contributor.authorFerreira, Vinícius
dc.contributor.authorPeng, Maoxiao
dc.contributor.authorZhang, Xushuai
dc.contributor.authorPower, Deborah
dc.date.accessioned2020-05-29T08:28:08Z
dc.date.available2020-05-29T08:28:08Z
dc.date.issued2020
dc.description.abstractBiomineralization is the process by which living organisms acquired the capacity to accumulate minerals in tissues. Shells are the biomineralized exoskeleton of marine molluscs produced by the mantle but factors that regulate mantle shell building are still enigmatic. This study sought to identify candidate regulatory factors of molluscan shell mineralization and targeted family B G-protein coupled receptors (GPCRs) and ligands that include calcium regulatory factors in vertebrates, such as calcitonin (CALC). In molluscs, CALC receptor (CALCR) number was variable and arose through lineage and species-specific duplications. The Mediterranean mussel (Mytilus galloprovincialis) mantle transcriptome expresses six CALCR-like and two CALC-precursors encoding four putative mature peptides. Mussel CALCR-like are activated in vitro by vertebrate CALC but only receptor CALCRIIc is activated by the mussel CALCIIa peptide (EC50 = 2.6 ×10-5 M). Ex-vivo incubations of mantle edge tissue and mantle cells with CALCIIa revealed they accumulated significantly more calcium than untreated tissue and cells. Mussel CALCIIa also significantly decreased mantle acid phosphatase activity, which is associated with shell remodelling. Our data indicate the CALC-like system as candidate regulatory factors of shell mineralization. The identification of the CALC system from molluscs to vertebrates suggests it is an ancient and conserved calcium regulatory system of mineralization.pt_PT
dc.description.sponsorshipCCMAR UIDB/04326/2020; CRESC Algarve 2020 and COMPETE 2020 through project EMBRC.PT ALG-01-0145-FEDER-022121. FCT: DL57/2016/CP1361/CT0020pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1038/s41598-020-64118-wpt_PT
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10400.1/13965
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectGene-related peptidept_PT
dc.subjectProteinspt_PT
dc.subjectResistant acid-phosphatasept_PT
dc.subjectBonept_PT
dc.subjectOyster pinctada-fucatapt_PT
dc.subjectReceptorspt_PT
dc.subjectOcean acidificationpt_PT
dc.subjectTeleost fishpt_PT
dc.titleThe calcitonin-like system is an ancient regulatory system of biomineralizationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue1pt_PT
oaire.citation.startPage7581pt_PT
oaire.citation.titleScientific Reportspt_PT
oaire.citation.volume10pt_PT
person.familyNameCardoso
person.familyNameFélix
person.familyNamePeng
person.familyNamePower
person.givenNameJoão
person.givenNameRute
person.givenNameMaoxiao
person.givenNameDeborah Mary
person.identifier14332
person.identifier.ciencia-id8B16-F203-2AFC
person.identifier.ciencia-idFB1A-1325-8E25
person.identifier.ciencia-id0A1D-96B9-27EB
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0001-7890-0170
person.identifier.orcid0000-0002-4144-3452
person.identifier.orcid0000-0002-4855-1202
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridM-4151-2013
person.identifier.ridM-6164-2013
person.identifier.scopus-author-id7201822956
person.identifier.scopus-author-id56006773000
person.identifier.scopus-author-id7101806760
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication1b670c84-15e3-4776-8871-50f9eb0eed0d
relation.isAuthorOfPublication9b5e820d-e41f-4c28-8832-823658d29ae9
relation.isAuthorOfPublication43d269d9-e043-4d27-b3ee-5f1f657dcd37
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscovery43d269d9-e043-4d27-b3ee-5f1f657dcd37

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
s41598-020-64118-w.pdf
Size:
3.63 MB
Format:
Adobe Portable Document Format
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)