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Extracellular electrophysiological measurements of cooperative signals in astrocytes populations

2017-10Journal article Open Access
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dc.contributor.authorMestre, Ana L. G.
dc.contributor.authorINÁCIO, PEDRO
dc.contributor.authorELAMINE, Youssef
dc.contributor.authorAsgarifar, Sanaz
dc.contributor.authorLourenco, Ana S.
dc.contributor.authorCristiano, Maria L. S.
dc.contributor.authorAguiar, Paulo
dc.contributor.authorMedeiros, Maria C. R.
dc.contributor.authorAraĂșjo, InĂȘs
dc.contributor.authorVentura, Joao
dc.contributor.authorGomes, Henrique L.
dc.date.accessioned2018-12-07T14:58:08Z
dc.date.available2018-12-07T14:58:08Z
dc.date.issued2017-10
dc.description.abstractAstrocytes are neuroglial cells that exhibit functional electrical properties sensitive to neuronal activity and capable of modulating neurotransmission. Thus, electrophysiological recordings of astroglial activity are very attractive to study the dynamics of glial signaling. This contribution reports on the use of ultra-sensitive planar electrodes combined with low noise and low frequency amplifiers that enable the detection of extracellular signals produced by primary cultures of astrocytes isolated from mouse cerebral cortex. Recorded activity is characterized by spontaneous bursts comprised of discrete signals with pronounced changes on the signal rate and amplitude. Weak and sporadic signals become synchronized and evolve with time to higher amplitude signals with a quasi-periodic behavior, revealing a cooperative signaling process. The methodology presented herewith enables the study of ionic fluctuations of population of cells, complementing the single cells observation by calcium imaging as well as by patch-clamp techniques.
dc.description.sponsorshipPortuguese Foundation for Science and Technology (FCT) [PTDC/EEI-AUT/5442/2014]; Instituto de Telecomunicacoes [UID/Multi/04326/2013]; Associated Laboratory - Institute of Nanoscience and Nanotechnology [POCI-01-0145-FEDER-016623]; [PTDC/CTM-NAN/3146/2014]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.3389/fncir.2017.00080
dc.identifier.issn1662-5110
dc.identifier.urihttp://hdl.handle.net/10400.1/11876
dc.language.isoeng
dc.peerreviewedyes
dc.publisherFrontiers Media Sa
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBergmann Glial-Cells
dc.subjectIn-Situ
dc.subjectIon channels
dc.subjectCalcium oscillations
dc.subjectNeurons
dc.subjectSodium
dc.subjectWaves
dc.subjectCommunication
dc.subjectHomeostasis
dc.subjectVivo
dc.titleExtracellular electrophysiological measurements of cooperative signals in astrocytes populations
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.citation.startPage80
oaire.citation.titleFrontiers in Neural Circuits
oaire.citation.volume11
oaire.fundingStream5876
person.familyNameINÁCIO
person.familyNameElamine
person.familyNameAsgarifar
person.familyNameLourenço
person.familyNameCristiano
person.familyNameAguiar
person.familyNamePombinho de AraĂșjo
person.familyNameGomes
person.givenNamePEDRO
person.givenNameYoussef
person.givenNameSanaz
person.givenNameAna Sofia
person.givenNameMaria de Lurdes
person.givenNamePaulo
person.givenNameInĂȘs Maria
person.givenNameHenrique Leonel
person.identifier2001885
person.identifier453174
person.identifierhttps://scholar.google.com/citations?hl=en&user=LFpifG0AAAAJ
person.identifier170713
person.identifierF-4703-2012
person.identifier.ciencia-id1510-2B36-5ED1
person.identifier.ciencia-id421E-A08C-57A2
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person.identifier.ciencia-idE411-6006-5A01
person.identifier.ciencia-id0413-0808-A59C
person.identifier.ciencia-idD11F-D4CA-2947
person.identifier.orcid0000-0003-1986-7918
person.identifier.orcid0000-0002-8650-1580
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person.identifier.orcid0000-0002-2438-0111
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person.identifier.ridG-2345-2012
person.identifier.scopus-author-id55938897300
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person.identifier.scopus-author-id36842157600
person.identifier.scopus-author-id56271084100
person.identifier.scopus-author-id7005305880
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a CiĂȘncia e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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