dc.contributor.author	Kuhl, H.
dc.contributor.author	Beck, Alfred
dc.contributor.author	Wozniak, Grzegorz
dc.contributor.author	Canario, Adelino V. M.
dc.contributor.author	Volckaert, F.
dc.contributor.author	Reinhardt, Richard
dc.date.accessioned	2012-02-14T22:28:25Z
dc.date.available	2012-02-14T22:28:25Z
dc.date.issued	2010-01-27
dc.date.updated	2012-02-06T16:13:37Z
dc.description.abstract	Abstract Background Food supply from the ocean is constrained by the shortage of domesticated and selected fish. Development of genomic models of economically important fishes should assist with the removal of this bottleneck. European sea bass Dicentrarchus labrax L. (Moronidae, Perciformes, Teleostei) is one of the most important fishes in European marine aquaculture; growing genomic resources put it on its way to serve as an economic model. Results End sequencing of a sea bass genomic BAC-library enabled the comparative mapping of the sea bass genome using the three-spined stickleback Gasterosteus aculeatus genome as a reference. BAC-end sequences (102,690) were aligned to the stickleback genome. The number of mappable BACs was improved using a two-fold coverage WGS dataset of sea bass resulting in a comparative BAC-map covering 87% of stickleback chromosomes with 588 BAC-contigs. The minimum size of 83 contigs covering 50% of the reference was 1.2 Mbp; the largest BAC-contig comprised 8.86 Mbp. More than 22,000 BAC-clones aligned with both ends to the reference genome. Intra-chromosomal rearrangements between sea bass and stickleback were identified. Size distributions of mapped BACs were used to calculate that the genome of sea bass may be only 1.3 fold larger than the 460 Mbp stickleback genome. Conclusions The BAC map is used for sequencing single BACs or BAC-pools covering defined genomic entities by second generation sequencing technologies. Together with the WGS dataset it initiates a sea bass genome sequencing project. This will allow the quantification of polymorphisms through resequencing, which is important for selecting highly performing domesticated fish.	por
dc.description.version	Peer Reviewed
dc.identifier.citation	BMC Genomics. 2010 Jan 27;11(1):68	por
dc.identifier.doi	http://dx.doi.org/10.1186/1471-2164-11-68
dc.identifier.other	AUT: ACA00258;
dc.identifier.uri	http://hdl.handle.net/10400.1/900
dc.language.iso	eng	por
dc.language.rfc3066	eng
dc.peerreviewed	yes	por
dc.rights.holder	Kuhl et al.; licensee BioMed Central Ltd.
dc.title	The European sea bass Dicentrarchus labrax genome puzzle: comparative BAC-mapping and low coverage shotgun sequencing	por
dc.type	journal article
dspace.entity.type	Publication
person.familyName	Canario
person.givenName	Adelino
person.identifier	143624
person.identifier.ciencia-id	1F1E-D3B3-F804
person.identifier.orcid	0000-0002-6244-6468
person.identifier.rid	C-7942-2009
person.identifier.scopus-author-id	56568523700
rcaap.rights	openAccess	por
rcaap.type	article	por
relation.isAuthorOfPublication	5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscovery	5f6e51ee-9113-469e-8b9e-f30f2d452521

The European sea bass Dicentrarchus labrax genome puzzle: comparative BAC-mapping and low coverage shotgun sequencing

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