Repository logo
 
Publication

Alternative transcripts of DMRT1 in the European sea bass: expression during gonadal differentiation

2009Journal article Restricted access
Simple item page
dc.contributor.authorDeloffre, Laurence A. M.
dc.contributor.authorMartins, Rute S. T.
dc.contributor.authorMylonas, Constantinos C.
dc.contributor.authorCanario, Adelino V. M.
dc.date.accessioned2014-05-08T13:43:56Z
dc.date.available2014-05-08T13:43:56Z
dc.date.issued2009
dc.date.updated2014-05-08T10:40:05Z
dc.description.abstractDMRT1 is conserved from invertebrates to human and has been implicated in sex differentiation and testis function in many organisms.We report the cloning of two DMRT1 transcripts, DMRT1a and DMRT1b, encoded by a single gene in the European sea bass, Dicentrarchus labrax, a teleost fish with polygenic sex determination influenced by temperature. DMRT1a and DMRT1b are specific to the testis and are present as transcripts of 2.7 and 4.6 kb, as determined by Northern blot, although the cloned cDNAs are 1.2 and 1.9 kb. DMRT1a, encodes a protein of 306-amino acids, of similar size to what has been found in other teleosts, while the DMRT1b encodes a unique protein which differ from DMRT1a by having a 26 amino acids insertion which separates the S and Y domains. This insertion is the result of an extra exon (exon 4), present in the genomes of teleosts but not in other vertebrate genomes. Furthermore, unlike in the European sea bass, the putative product of DMRT1b in other teleost species is a truncated peptide. In European sea bass, the two transcripts are expressed at a similar level and have a similar expression profile in developing gonads. They are first detected by reverse transcription-polymerase chain reaction at 150 days post-hatch coinciding with the start of sex differentiation. After 200 days, expression increases in testis and decreases in ovary. Although it is not clear whether the two transcripts have differential roles it is suggested that both are required for testicular function.por
dc.identifier.citationDeloffre, Laurence A.M.; Martins, Rute S.T.; Mylonas, Constantinos C.; Canario, Adelino V.M. Alternative transcripts of DMRT1 in the European sea bass: Expression during gonadal differentiation, Aquaculture, 293, 1-2, 89-99, 2009.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.aquaculture.2009.03.048
dc.identifier.issn0044-8486
dc.identifier.otherAUT: ACA00258;
dc.identifier.urihttp://hdl.handle.net/10400.1/3812
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectDMRT1por
dc.subjectSex differentiationpor
dc.subjectDicentrarchus labraxpor
dc.subjectAlternative splicingpor
dc.subjectGonadal developmentpor
dc.titleAlternative transcripts of DMRT1 in the European sea bass: expression during gonadal differentiationpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage99por
oaire.citation.issue1-2por
oaire.citation.startPage89por
oaire.citation.titleAquaculturepor
oaire.citation.volume293por
person.familyNameCanario
person.givenNameAdelino
person.identifier143624
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id56568523700
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscovery5f6e51ee-9113-469e-8b9e-f30f2d452521

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Alternative transcripts of DMRT1 in the European sea bass Expression during gonadal differentiation.pdf
Size:
2.43 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.61 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)