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Cartilage acidic protein a novel therapeutic factor to improve skin damage repair?

2021-10Journal article Open access
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dc.contributor.authorFélix, Rute
dc.contributor.authorAnjos, Liliana
dc.contributor.authorCosta, Rita
dc.contributor.authorLetsiou, Sophia
dc.contributor.authorPower, Deborah Mary
dc.date.accessioned2021-11-12T16:00:05Z
dc.date.available2021-11-12T16:00:05Z
dc.date.issued2021-10
dc.description.abstractFish skin has been gaining attention due to its efficacy as a human-wound-treatment product and to identify factors promoting its enhanced action. Skin fibroblasts have a central role in maintaining skin integrity and secrete extra cellular matrix (ECM) proteins, growth factors and cytokines to rapidly repair lesions and prevent further damage or infection. The effects on scratch repair of the ubiquitous but poorly characterized ECM protein, cartilage acidic protein 1 (CRTAC1), from piscine and human sources were compared using a zebrafish SJD.1 primary fibroblast cell line. A classic in vitro cell scratch assay, immunofluorescence, biosensor and gene expression analysis were used. Our results demonstrated that the duplicate sea bass Crtac1a and Crtac1b proteins and human CRTAC-1A all promoted SJD.1 primary fibroblast migration in a classic scratch assay and in an electric cell impedance sensing assay. The immunofluorescence analysis revealed that CRTAC1 enhanced cell migration was most likely caused by actin-driven cytoskeletal changes and the cellular transcriptional response was most affected in the early stage (6 h) of scratch repair. In summary, our results suggest that CRTAC1 may be an important factor in fish skin promoting damage repair.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/md19100541pt_PT
dc.identifier.eissn1660-3397
dc.identifier.urihttp://hdl.handle.net/10400.1/17325
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectElectric cell impedance sensing (ECIS)pt_PT
dc.subjectFish skin fibroblastpt_PT
dc.subjectScratch assaypt_PT
dc.subjectVertebrate CRTAC1pt_PT
dc.subjectZebrafishpt_PT
dc.titleCartilage acidic protein a novel therapeutic factor to improve skin damage repair?pt_PT
dc.title.alternativeProteína ácida de cartilagem um novo fator terapêutico para melhorar o reparo de danos na pele?pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue10pt_PT
oaire.citation.startPage541pt_PT
oaire.citation.titleMarine Drugspt_PT
oaire.citation.volume19pt_PT
person.familyNameFélix
person.familyNameAnjos Guerreiro
person.familyNameCosta
person.familyNamePower
person.givenNameRute
person.givenNameLiliana Isabel Tomé
person.givenNameRita
person.givenNameDeborah Mary
person.identifierAAK-3800-2020
person.identifier.ciencia-idFB1A-1325-8E25
person.identifier.ciencia-id6D18-2D1C-A45C
person.identifier.ciencia-id8219-DFF3-929F
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0002-4144-3452
person.identifier.orcid0000-0001-9474-522X
person.identifier.orcid0000-0002-6975-7576
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridM-6164-2013
person.identifier.ridO-9136-2018
person.identifier.scopus-author-id56006773000
person.identifier.scopus-author-id8575757700
person.identifier.scopus-author-id57097731700
person.identifier.scopus-author-id7101806760
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication9b5e820d-e41f-4c28-8832-823658d29ae9
relation.isAuthorOfPublication721b5dca-ad42-40f8-9731-517eb5db009e
relation.isAuthorOfPublication943d1248-7839-42b3-89a9-b539b3209cd7
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscovery721b5dca-ad42-40f8-9731-517eb5db009e

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