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Identification of Sparus aurata bone morphogenetic protein 2: Molecular cloning, gene expression and in silico analysis of protein conserved features in vertebrates

2006-12Journal article Restricted access
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dc.contributor.authorRafael, Marta S.
dc.contributor.authorLaizé, Vincent
dc.contributor.authorCancela, Leonor
dc.date.accessioned2014-06-14T09:57:09Z
dc.date.available2014-06-14T09:57:09Z
dc.date.issued2006-12
dc.date.updated2014-06-03T09:58:15Z
dc.description.abstractBone morphogenetic protein 2 (BMP-2) is a secreted signaling molecule that acts as an inducer of bone formation and a regulator of embryonic development. The objectives of this work were as follows: (1) to clone the full-length cDNA of BMP-2 in a marine fish model, (2) analyze its gene expression during development, in adult tissues and in cell lines, and (3) identify protein conserved features of vertebrate BMP-2. Using a combination of RT- and 5′-RACE-PCR, a 1653-bp fragment corresponding to Sparus aurata BMP-2 cDNA (SaBMP-2) was amplified. Levels of SaBMP-2 gene expression were estimated using quantitative real-time PCR and shown to be strongly increased (150-fold induction) at gastrulation, thus suggesting a key role for BMP-2 in fish development. Tissue distribution of SaBMP-2 mRNA revealed highest levels in the calcified tissues bone, caudal fin and scales and in liver. BMP-2 was also found to be highly expressed in S. aurata bone-derived cell lines VSa13 and VSa16 and to be up-regulated (more than 10-fold induction) in mineralized VSa13 chondrocyte-like cells. Using bioinformatic tools and all vertebrate protein sequences available, conserved features of BMP-2 were characterized. The mature protein was shown to be highly conserved across 20 species indicating that BMP-2 function has been conserved throughout evolution, a finding that is in agreement with the widely accepted view of the important role played by BMPs in vertebrate development.por
dc.identifier.citationRafael MS; Laizé V; Cancela ML. Identification of Sparus aurata bone morphogenetic protein 2: Molecular cloning, gene expression and in silico analysis of protein conserved features in vertebrates, Bone, 39, 6, 1373-1381, 2006.por
dc.identifier.doihttp://dx.doi.org/ 10.1016/j.bone.2006.06.021
dc.identifier.issn8756-3282
dc.identifier.otherAUT: LCA00739;
dc.identifier.urihttp://hdl.handle.net/10400.1/4316
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherInternational Bone and Mineral Societypor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S8756328206005618por
dc.subjectBone morphogenetic proteinpor
dc.subjectBMP-2por
dc.subjectMarine teleost gilthead seabream [Sparus aurata]por
dc.subjectEmbryonic developmentpor
dc.subjectCalcified tissuespor
dc.titleIdentification of Sparus aurata bone morphogenetic protein 2: Molecular cloning, gene expression and in silico analysis of protein conserved features in vertebratespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1381por
oaire.citation.issue6por
oaire.citation.startPage1373por
oaire.citation.titleBonepor
oaire.citation.volume39por
person.familyNameRafael
person.familyNameLaizé
person.familyNameCancela
person.givenNameMarta
person.givenNameVincent
person.givenNameM. Leonor
person.identifier.ciencia-id1C10-BF17-0DD7
person.identifier.orcid0000-0002-5518-9790
person.identifier.orcid0000-0001-9565-9198
person.identifier.orcid0000-0003-3114-6662
person.identifier.ridB-4463-2008
person.identifier.scopus-author-id15078212800
person.identifier.scopus-author-id6602982778
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicatione4c6f8da-c3f6-4eee-bf78-f023843c343b
relation.isAuthorOfPublicationb51cd3d5-7ee4-47d8-9bc4-b3e297aa222f
relation.isAuthorOfPublicationb9bbfe32-3dfe-4131-ad14-a4394008447f
relation.isAuthorOfPublication.latestForDiscoveryb9bbfe32-3dfe-4131-ad14-a4394008447f

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