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Cryopreservation did not affect spermatogonia global methylation profile in Senegalese sole (Solea senegalensis)

dc.contributor.authorAlmeida, Maria Mafalda
dc.contributor.authorCabrita, Elsa
dc.contributor.authorLaizé, Vincent
dc.contributor.authorBrionne, Aurélien
dc.contributor.authorLabbé, Catherine
dc.contributor.authorFatsini Fernández, Elvira
dc.date.accessioned2025-02-04T11:39:56Z
dc.date.available2025-02-04T11:39:56Z
dc.date.issued2025-03
dc.description.abstractSpermatogonia cryopreservation is a method to preserve valuable genomes from both maternal and paternal origin. The damage associated with the application of this technology on post-thaw cell quality is important to assess, including at the epigenetic level. This study aimed to assess post-thawed spermatogonia quality by evaluating alterations in plasma membrane integrity, DNA integrity (fragmentation and apoptosis), lipid peroxidation (malondialdehyde levels) and epigenetic modifications (DNA methylation profile). We observed that plasma membrane integrity (fresh 78.98 % f 5.66; cryopreserved 62.81 % f 3.25; P = 0.003) and DNA integrity (fresh 32.95% f 2.28; cryopreserved 37.28% f 1.87; P = 0.0026) were affected by cryopreservation, while no difference in lipid peroxidation was observed (fresh 1.13 % f 0.45; cryopreserved 0.91 % f 0.96; P = 0.701). While global levels of DNA methylation were unaffected by cryopreservation (fresh 82.80 % f 0.47; cryopreserved 83.32 % f 0.81; P = 0.745), some differentially methylated cytosines (DMC) were observed in cryopreserved versus fresh spermatogonia (156 DMC). This study showed that spermatogonia cryopreserved according to our protocol provides a good supply of undamaged cells for several applications. The significance of the few detected DMCs deserves further attention since it may affect gamete differentiation and epigenetic profile.eng
dc.description.sponsorship3.0H2020-INFRAIA-2018-2020/H2020-INFRAIA-2019-1- 871108;
dc.identifier.doi10.1016/j.cryobiol.2024.105162
dc.identifier.issn0011-2240
dc.identifier.urihttp://hdl.handle.net/10400.1/26738
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relationThe imprinted characteristics of spermatogonia antioxidant capacity and their role in oxidative stress tolerance
dc.relationMitigation solutions for the reproductive disorder of Senegalese sole cultivation – MITIGASOLE
dc.relation.ispartofCryobiology
dc.rights.uriN/A
dc.subjectGerm cells
dc.subjectPlasma membrane integrity
dc.subjectDNA integrity
dc.subjectLipid peroxidation
dc.subjectDNA methylation
dc.titleCryopreservation did not affect spermatogonia global methylation profile in Senegalese sole (Solea senegalensis)eng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardTitleThe imprinted characteristics of spermatogonia antioxidant capacity and their role in oxidative stress tolerance
oaire.awardTitleMitigation solutions for the reproductive disorder of Senegalese sole cultivation – MITIGASOLE
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/EXPL%2FCVT-CVT%2F0305%2F2021/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 3ed/2020.04181.CEECIND%2FCP1597%2FCT0002/PT
oaire.citation.startPage105162
oaire.citation.titleCryobiology: International Journal of Low Temperature Biology and Medicine
oaire.citation.volume118
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream3599-PPCDT
oaire.fundingStreamCEEC IND 3ed
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameAlmeida
person.familyNameCabrita
person.familyNameLaizé
person.familyNameFatsini Fernández
person.givenNameMaria Mafalda
person.givenNameElsa
person.givenNameVincent
person.givenNameElvira
person.identifierhttps://scholar.google.es/citations?hl=en&pli=1&user=55mbsZIAAAAJ
person.identifier.ciencia-idEA1D-0571-7A6D
person.identifier.orcid0000-0003-0192-761X
person.identifier.orcid0000-0003-3864-8841
person.identifier.orcid0000-0001-9565-9198
person.identifier.orcid0000-0003-0371-5290
person.identifier.ridE-8523-2012
person.identifier.ridB-4463-2008
person.identifier.scopus-author-id26645697000
person.identifier.scopus-author-id6602982778
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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