Publication
Early disruption of the actin cytoskeleton in cultured cerebellar granule neurons exposed to 3-morpholinosydnonimine-oxidative stress is linked to alterations of the cytosolic calcium concentration
| dc.contributor.author | Tiago, Teresa | |
| dc.contributor.author | Marques-da-Silva, Dorinda | |
| dc.contributor.author | Samhan-Arias, A. K. | |
| dc.contributor.author | Aureliano, M. | |
| dc.contributor.author | Gutiérrez-Merino, Carlos | |
| dc.date.accessioned | 2012-06-27T09:46:41Z | |
| dc.date.available | 2012-06-27T09:46:41Z | |
| dc.date.issued | 2011 | |
| dc.description.abstract | Cytoskeleton damage is a frequent feature in neuronal cell death and one of the early events in oxidantinduced cell injury. This work addresses whether actin cytoskeleton reorganization is an early event of SIN-1-induced extracellular nitrosative/oxidative stress in cultured cerebellar granule neurons (CGN). The actin polymerization state, i.e. the relative levels of G-/F-actin, was quantitatively assessed by the ratio of the fluorescence intensities of microscopy images obtained from CGN double-labelled with Alexa594- DNase-I (for actin monomers) and Bodipy-FL-phallacidin (for actin filaments). Exposure ofCGNto a flux of peroxynitrite as low as 0.5–1 M/min during 30 min (achieved with 0.1mMSIN-1) was found to promote alterations of the actin cytoskeleton dynamics as it increases the G-actin/F-actin ratio. Because L-type voltage-operated Ca2+ channels (L-VOCC) are primary targets in CGN exposed to SIN-1, the possible role of Ca2+ dynamics on the perturbation of the actin cytoskeleton was also assessed from the cytosolic Ca2+ concentration response to the L-VOCC’s agonist FPL-64176 and to the L-VOCC’s blocker nifedipine. The results showed that SIN-1 induced changes in the actin polymerization state correlated with its ability to decrease Ca2+ influx through L-VOCC. Combined analysis of cytosolic Ca2+ concentration and G-actin/Factin ratio alterations by SIN-1, cytochalasin D, latrunculin B and jasplakinolide support that disruption of the actin cytoskeleton is linked to cytosolic calcium concentration changes. | por |
| dc.identifier.issn | 0143-4160 | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/1323 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Elsevier | por |
| dc.subject | Neurons | por |
| dc.subject | Stress | por |
| dc.title | Early disruption of the actin cytoskeleton in cultured cerebellar granule neurons exposed to 3-morpholinosydnonimine-oxidative stress is linked to alterations of the cytosolic calcium concentration | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 183 | por |
| oaire.citation.issue | 49 | por |
| oaire.citation.startPage | 174 | por |
| oaire.citation.title | Cell Calcium | por |
| person.familyName | Aureliano | |
| person.familyName | Gutierrez-Merino | |
| person.givenName | Manuel | |
| person.givenName | Carlos | |
| person.identifier | 584146 | |
| person.identifier.ciencia-id | AA14-3490-DC5E | |
| person.identifier.orcid | 0000-0003-4858-3201 | |
| person.identifier.orcid | 0000-0003-3673-7007 | |
| person.identifier.rid | I-3283-2012 | |
| person.identifier.rid | K-4574-2014 | |
| person.identifier.scopus-author-id | 6603412860 | |
| rcaap.rights | restrictedAccess | por |
| rcaap.type | article | por |
| relation.isAuthorOfPublication | bb413661-7edd-4b57-8338-33889cfd05db | |
| relation.isAuthorOfPublication | 1edd3bda-28a9-4d7f-a404-f867f72ddefa | |
| relation.isAuthorOfPublication.latestForDiscovery | 1edd3bda-28a9-4d7f-a404-f867f72ddefa |