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In vitro metabolism of pregnenolone to 7 alpha-hydroxypregnenolone by rainbow trout embryos

2003Journal article Restricted access
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dc.contributor.authorPetkam, R.
dc.contributor.authorRenaud, R. L.
dc.contributor.authorFreitas, A. M. M. S.
dc.contributor.authorCanario, Adelino V. M.
dc.contributor.authorRaeside, J. I.
dc.contributor.authorKime, D. E.
dc.contributor.authorLeatherland, J. F.
dc.date.accessioned2014-05-13T16:12:15Z
dc.date.available2014-05-13T16:12:15Z
dc.date.issued2003
dc.date.updated2014-05-13T10:17:10Z
dc.description.abstractTissues taken from rainbow trout embryos at several developmental stages, were incubated in the presence of radioactively-labelled pregnenolone in order to determine the capability of salmonid embryos to metabolize steroids, such as pregnenolone, that are incorporated into the oocyte during gonadal growth and maturation. High performance liquid chromatography was used to separate the steroid products, and gas chromatography–mass spectrometry was applied for the chemical identification of the product. 7α-Hydroxypregnenolone, previously known to be produced only by ovarian tissues, was found to be the sole metabolite of pregnenolone metabolism by rainbow trout embryos. Sulfate and glucuronide conjugated forms of 7α-hydroxypregnenolone were also produced. We hypothesize that this metabolite provides a pathway for excretion of pregnenolone, enabling the embryo to maintain its own steroid milieu, although the possibility of 7α-hydroxypregnenolone also playing a physiological role cannot be excluded.por
dc.identifier.citationPetkam, R.; Renaud, R. L.; Freitas, Amms; Canario, A. V. M.; Raeside, J. I.; Kime, D. E.; Leatherland, J. F.In vitro metabolism of pregnenolone to 7 alpha-hydroxypregnenolone by rainbow trout embryos, General and Comparative Endocrinology, 131, 3, 241-249, 2003por
dc.identifier.otherAUT: ACA00258; AFR00371;
dc.identifier.urihttp://hdl.handle.net/10400.1/3911
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectGas chromatography and mass spectrometrypor
dc.subjectHigh performance liquid chromatographypor
dc.subjectGonadal steroidspor
dc.titleIn vitro metabolism of pregnenolone to 7 alpha-hydroxypregnenolone by rainbow trout embryospor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage249por
oaire.citation.startPage241por
oaire.citation.titleGeneral and Comparative Endocrinologypor
oaire.citation.volume131por
person.familyNameCanario
person.givenNameAdelino
person.identifier143624
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id56568523700
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscovery5f6e51ee-9113-469e-8b9e-f30f2d452521

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