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Mapping of a locus for adult plant resistance to downy mildew in broccoli (Brassica oleracea convar. italica)

2004Journal article Restricted access
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dc.contributor.authorFarinho, M.
dc.contributor.authorCoelho, P.
dc.contributor.authorCarlier, Jorge
dc.contributor.authorSvetleva, D.
dc.contributor.authorMonteiro, A.
dc.contributor.authorLeitão, J. M.
dc.date.accessioned2015-06-19T14:36:28Z
dc.date.available2015-06-19T14:36:28Z
dc.date.issued2004
dc.description.abstractThe identification of the gene Pp523, conferring downy mildew resistance to adult plants of broccoli (Brassica oleracea convar. italica), led to the construction of a genetic map that included this resistance locus, 301 amplified fragment length polymorphisms, 55 random amplified polymorphic DNAs, 46 inter-simple sequence repeats, three simple sequence repeats, four other PCR markers and a flower colour locus, all gathered into nine major linkage groups. Nineteen additional molecular markers were clustered into one group of four markers, one group of three markers and six pairs of markers. The map spans over 731.9 cM, corresponding to 89.5% of the 818 cM estimated to be the total genome length. A significant number of the mapped markers, 19.3%, showed distorted segregation. The average distance between mapped adjacent markers is 1.64 cM, which places this map among the densest published to date for this species. Using bulked segregant analysis, we identified a group of molecular markers flanking and closely linked in coupling to the resistance gene and included these in the map. Two markers linked in coupling, OPK17_980 and AT.CTA_133/134, are located at 3.1 cM and 3.6 cM, respectively, at each side from the resistance gene. These markers can be used for marker-assisted selection in breeding programs aiming at the introgression of this gene in susceptible B. oleracea genotypes. The fine mapping of the genomic region surrounding the Pp523 resistance gene is currently being carried out, a basic condition for its isolation via positional cloning.
dc.identifier.doihttps://dx.doi.org/10.1007/s00122-004-1747-0
dc.identifier.issn0040-5752
dc.identifier.otherAUT: JLE00446;
dc.identifier.urihttp://hdl.handle.net/10400.1/6520
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer Verlag
dc.relation.isbasedonP-000-81D
dc.titleMapping of a locus for adult plant resistance to downy mildew in broccoli (Brassica oleracea convar. italica)
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1398
oaire.citation.startPage1392
oaire.citation.titleTAG Theoretical and Applied Genetics
oaire.citation.volume109
person.familyNameCarlier
person.familyNameLeitão
person.givenNameJorge
person.givenNameJosé
person.identifier258398
person.identifier.ciencia-id691D-A1BB-15CC
person.identifier.ciencia-id7A17-3E0A-73AD
person.identifier.orcid0000-0003-0675-2716
person.identifier.orcid0000-0003-4278-0415
person.identifier.ridAAH-3958-2020
person.identifier.scopus-author-id7102166949
person.identifier.scopus-author-id7005119796
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublicationd4af2b00-61d6-4fd9-b05d-9ed235b5c7de
relation.isAuthorOfPublication0f990d1c-2913-4f8a-8fb6-f8b02721247f
relation.isAuthorOfPublication.latestForDiscovery0f990d1c-2913-4f8a-8fb6-f8b02721247f

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