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Targeted gene disruption coupled with metabolic screen approach to uncover the LEAFY COTYLEDON1-LIKE4 (L1L4) function in tomato fruit metabolism

2017-07Journal article Open access
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dc.contributor.authorGago, Custódia
dc.contributor.authorDrosou, Victoria
dc.contributor.authorPaschalidis, Konstantinos
dc.contributor.authorGuerreiro, Adriana
dc.contributor.authorMiguel, Maria Graca
dc.contributor.authorAntunes, Maria Dulce
dc.contributor.authorHilioti, Zoe
dc.date.accessioned2019-11-20T15:07:31Z
dc.date.available2019-11-20T15:07:31Z
dc.date.issued2017-07
dc.description.abstractKey message Functional analysis of tomato L1L4 master transcription factor resulted in important metabolic changes affecting tomato fruit quality. Abstract Tomato fruits from mutant lines bearing targeted disruption of the heterotrimeric nuclear transcription factor Y (NF-Y) transcription factor (TF) gene LEAFY-COTYLEDON1-LIKE4 (L1L4, NF-YB6), a master regulator of biosynthesis for seed storage proteins and fatty acids, were evaluated for metabolites content and morphology. Metabolic screens using LC-MS/MS-based analysis and physico-chemical methods in different L1L4 mutants of the fourth generation allowed a comparative assessment of the effects of the TF disruption. Mutagenesis resulted in fruits phenotypically similar to wild-type with subtle shape differences in the distal end protrusion and symmetry. Conversely, mutant fruits from independent lines had significant variation in moisture content, titratable acidity and overall metabolite profiles including oxalic and citric acid, fructose, beta-carotene, total polyphenols and antioxidants. Lines 6, 7 and 9 were the richest in beta-carotene and antioxidant activity, line 4 in ascorbic acid and lines 4 and 8 in succinic acid. The reduced content of the anti-nutrient oxalic acid in several mutant fruits suggests that L1L4 gene may regulate the accumulation of this compound during fruit development. Detailed LC-MS/MS analysis of mutant seeds showed substantial differences in bioactive compounds compared to wild-type seeds. Taken together, the results suggest that the L1L4 TF is a significant regulator of metabolites both in tomato fruit and seeds providing a molecular target for crop improvement. Elucidation of the candidate genes encoding key enzymes in the affected metabolic pathways aimed to facilitate the L1L4 gene network exploration and eventually lead to systems biology approaches in tomato fruit quality.
dc.description.sponsorshipEuropean funded COST ACTION [FA1106]
dc.identifier.doi10.1007/s00299-017-2137-9
dc.identifier.issn0721-7714
dc.identifier.issn1432-203X
dc.identifier.urihttp://hdl.handle.net/10400.1/13076
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer
dc.subjectQuantitative trait loci
dc.subjectCcaat-binding factor
dc.subjectOrganic-acid content
dc.subjectFactor Nf-Y
dc.subjectLycopersicon-esculentum
dc.subjectAntioxidant activity
dc.subjectTranscription factor
dc.subjectLycopene content
dc.subjectQtl analysis
dc.subjectBiochemical-analysis
dc.titleTargeted gene disruption coupled with metabolic screen approach to uncover the LEAFY COTYLEDON1-LIKE4 (L1L4) function in tomato fruit metabolism
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1082
oaire.citation.issue7
oaire.citation.startPage1065
oaire.citation.titlePlant Cell Reports
oaire.citation.volume36
person.familyNameGago
person.familyNameGuerreiro
person.familyNameMiguel
person.familyNameAntunes
person.givenNameCustódia
person.givenNameAdriana
person.givenNameMaria
person.givenNameMaria Dulce
person.identifierR-004-VY7
person.identifier177556
person.identifier.ciencia-id6517-A4D9-05AD
person.identifier.ciencia-id5318-F751-754D
person.identifier.ciencia-idC11B-9B05-217E
person.identifier.orcid0000-0001-9914-3615
person.identifier.orcid0000-0002-3380-3892
person.identifier.orcid0000-0002-1601-5501
person.identifier.orcid0000-0002-8913-6136
person.identifier.ridG-8496-2019
person.identifier.ridA-4683-2012
person.identifier.scopus-author-id37060829600
person.identifier.scopus-author-id56007053600
person.identifier.scopus-author-id9941792100
person.identifier.scopus-author-id7102645075
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication52857c66-1fbd-4c23-ab8e-83bd6df478f5
relation.isAuthorOfPublication08f4d8b1-7e3f-4c60-80b2-f6bffcb71878
relation.isAuthorOfPublicationecda84a3-0649-4504-abb0-9c4c56ff862c
relation.isAuthorOfPublication7947cc50-4ae0-4ada-8ddf-081f247adc90
relation.isAuthorOfPublication.latestForDiscovery08f4d8b1-7e3f-4c60-80b2-f6bffcb71878

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