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Identification of an osteocalcin isoform in fish with a large acidic prodomain

2006-06Journal article Open Access
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dc.contributor.authorLaizé, Vincent
dc.contributor.authorS B Viegas, Carla
dc.contributor.authorPrice, P. A.
dc.contributor.authorCancela, Leonor
dc.date.accessioned2014-06-13T10:15:17Z
dc.date.available2014-06-13T10:15:17Z
dc.date.issued2006-06
dc.date.updated2014-06-03T09:57:39Z
dc.description.abstractOsteocalcin is a small, secreted bone protein whose gene consists of four exons. In the course of analyzing the structure of fish osteocalcin genes, we recently found that the spotted green pufferfish has two possible exon 2 structures, one of 15 bp and the other of 324 bp. Subsequent analysis of the pufferfish cDNA showed that only the transcript with a large exon 2 exists. Exon 2 codes for the osteocalcin propeptide, and exon 2 of pufferfish osteocalcin is ∼3.4-fold larger than exon 2 previously found in other vertebrate species. We have termed this new pufferfish osteocalcin isoform OC2. Additional studies showed that the OC2 isoform is restricted to a unique fish taxonomic group, the Osteichthyes; OC2 is the only osteocalcin isoform found so far in six Osteichthyes species, whereas both OC1 and OC2 isoforms coexist in zebrafish and rainbow trout. The larger size of the OC2 propeptide is due to an acidic region that is likely to be highly phosphorylated and has no counterpart in the OC1 propeptide. We propose 1) that OC1 and OC2 are encoded by distinct genes that originated from a duplication event that probably occurred in the teleost fish lineage soon after divergence from tetrapods and 2) that the novel OC2 propeptide could be, if secreted, a phosphoprotein that participates in the regulation of biomineralization through its large acidic and phosphorylated propeptide.por
dc.identifier.citationLaizé V; Viegas CSB; Price PA; Cancela ML. Identification of an osteocalcin isoform in fish with a large acidic prodomain, Journal of Biological Chemistry, 281, 22, 15037-15043, 2006.por
dc.identifier.doihttp://dx.doi.org/ 10.1074/jbc.M600373200
dc.identifier.issn0021-9258
dc.identifier.otherAUT: LCA00739
dc.identifier.urihttp://hdl.handle.net/10400.1/4296
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherThe American Society for Biochemistry and Molecular Biologypor
dc.relation.publisherversionhttp://www.jbc.org/content/281/22/15037.longpor
dc.subjectOsteocalcin Isoformpor
dc.subjectAcidic Prodomainpor
dc.titleIdentification of an osteocalcin isoform in fish with a large acidic prodomainpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage15043por
oaire.citation.issue22por
oaire.citation.startPage15037por
oaire.citation.titleJournal of Biological Chemistrypor
oaire.citation.volume28por
person.familyNameLaizé
person.familyNameViegas
person.familyNameCancela
person.givenNameVincent
person.givenNameCarla
person.givenNameM. Leonor
person.identifier.ciencia-idC414-F596-EEC6
person.identifier.orcid0000-0001-9565-9198
person.identifier.orcid0000-0002-5765-3665
person.identifier.orcid0000-0003-3114-6662
person.identifier.ridB-4463-2008
person.identifier.ridN-6695-2014
person.identifier.scopus-author-id6602982778
person.identifier.scopus-author-id8656310300
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationb51cd3d5-7ee4-47d8-9bc4-b3e297aa222f
relation.isAuthorOfPublication33984b3c-ffac-477a-896a-ddbf7aced4e3
relation.isAuthorOfPublicationb9bbfe32-3dfe-4131-ad14-a4394008447f
relation.isAuthorOfPublication.latestForDiscoveryb9bbfe32-3dfe-4131-ad14-a4394008447f

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