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Macroalgal–bacterial interactions: identification and role of thallusin in morphogenesis of the seaweed Ulva (Chlorophyta)

2020Journal article Open access
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dc.contributor.authorWichard, Thomas
dc.contributor.authorUlrich, Johann F
dc.contributor.authorMohr, Jan Frieder
dc.contributor.authorKwantes, Michiel
dc.contributor.authorEngelen, Aschwin
dc.contributor.authorWeiss, Anne
dc.contributor.authorGrueneberg, Jan
dc.contributor.authorDeicke, Michael
dc.contributor.authorCalifano, Gianmaria
dc.contributor.authorAlsufyani, Taghreed
dc.date.accessioned2020-07-16T14:51:15Z
dc.date.available2020-07-16T14:51:15Z
dc.date.issued2020
dc.description.abstractMacroalgal microbiomes have core functions related to biofilm formation, growth, and morphogenesis of seaweeds. In particular, the growth and development of the sea lettuce Ulva spp. (Chlorophyta) depend on bacteria releasing morphogenetic compounds. Under axenic conditions, the macroalga Ulva mutabilis develops a callus-like phenotype with cell wall protrusions. However, co-culturing with Roseovarius sp. (MS2) and Maribacter sp. (MS6), which produce various stimulatory chemical mediators, completely recovers morphogenesis. This ecological reconstruction forms a tripartite community which can be further studied for its role in cross-kingdom interactions. Hence, our study sought to identify algal growth- and morphogenesis-promoting factors (AGMPFs) capable of phenocopying the activity of Maribacter spp. We performed bioassay-guided solid-phase extraction in water samples collected from U. mutabilis aquaculture systems. We uncovered novel ecophysiological functions of thallusin, a sesquiterpenoid morphogen, identified for the first time in algal aquaculture. Thallusin, released by Maribacter sp., induced rhizoid and cell wall formation at a concentration of 11 pmol l-1. We demonstrated that gametes acquired the iron complex of thallusin, thereby linking morphogenetic processes with intracellular iron homeostasis. Understanding macroalgae-bacteria interactions permits further elucidation of the evolution of multicellularity and cellular differentiation, and development of new applications in microbiome-mediated aquaculture systems.pt_PT
dc.description.sponsorshipFCT: UID/Multi/04326/2019; UIDB/04326/2020; CCMAR/ID/16/2018; CEECINST/00114/2018. German Research Foundation (DFG) CRC1127; European Union (EU) 642575; ASSEMBLE 227799; COST Action 'Phycomorph' FA1406.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1093/jxb/eraa066pt_PT
dc.identifier.issn0022-0957
dc.identifier.urihttp://hdl.handle.net/10400.1/14096
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationThe Algal Microbiome: Friends and Foes
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAlgal growthpt_PT
dc.subjectRhizoidpt_PT
dc.subjectCell wallpt_PT
dc.subjectPhytohormonept_PT
dc.subjectCross-kingdom interactionpt_PT
dc.subjectMorphogenesis-promoting factorpt_PT
dc.subjectMorphogenesispt_PT
dc.subjectSiderophorept_PT
dc.subjectSeaweedpt_PT
dc.titleMacroalgal–bacterial interactions: identification and role of thallusin in morphogenesis of the seaweed Ulva (Chlorophyta)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleThe Algal Microbiome: Friends and Foes
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/642575/EU
oaire.citation.endPage3349pt_PT
oaire.citation.issue11pt_PT
oaire.citation.startPage3340pt_PT
oaire.citation.titleJournal of Experimental Botanypt_PT
oaire.citation.volume71pt_PT
oaire.fundingStreamH2020
person.familyNameEngelen
person.familyNameCalifano
person.givenNameAschwin
person.givenNameGianmaria
person.identifier.ciencia-id911A-9A0C-744D
person.identifier.ciencia-id5B13-85E1-8549
person.identifier.orcid0000-0002-9579-9606
person.identifier.orcid0000-0003-1868-3738
person.identifier.ridM-3432-2013
person.identifier.scopus-author-id6701622770
person.identifier.scopus-author-id57192871486
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication33d5a223-f2c9-4c68-984f-9213f15a05b0
relation.isAuthorOfPublication50da6b43-f6de-4ecf-a0c8-51651c2ffd86
relation.isAuthorOfPublication.latestForDiscovery33d5a223-f2c9-4c68-984f-9213f15a05b0
relation.isProjectOfPublication1912e70c-3b72-4eb9-94ff-0142b5ad01df
relation.isProjectOfPublication.latestForDiscovery1912e70c-3b72-4eb9-94ff-0142b5ad01df

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