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Effects of outdoor and indoor cultivation on the polar lipid composition and antioxidant activity of Nannochloropsis oceanica and Nannochloropsis limnetica: A lipidomics perspective

2022-05Journal article Restricted access
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dc.contributor.authorCouto, Daniela
dc.contributor.authorConde, Tiago A.
dc.contributor.authorMelo, Tânia
dc.contributor.authorNeves, Bruna
dc.contributor.authorCosta, Margarida
dc.contributor.authorCunha, Pedro
dc.contributor.authorGuerra, Inês
dc.contributor.authorCorreia, Nádia
dc.contributor.authorSilva, Joana T.
dc.contributor.authorPereira, Hugo
dc.contributor.authorVarela, João
dc.contributor.authorSilva, Joana
dc.contributor.authorDomingues, Rosário
dc.contributor.authorDomingues, Pedro
dc.date.accessioned2022-10-28T13:36:41Z
dc.date.available2022-10-28T13:36:41Z
dc.date.issued2022-05
dc.description.abstractNannochloropsis is a genus of eicosapentaenoic acid-rich microalgae with high levels of value-added polar lipids. However, the polar lipid composition of microalgal biomass is highly dependent on culture conditions (e.g., light or temperature), which are significantly different under indoor and outdoor culture conditions. In this study, we sought to investigate the plasticity of the polar lipid profile of a marine (N. oceanica) and a freshwater (N. limnetica) species of Nannochloropsis grown in indoor and outdoor photobioreactors. To this end, the polar lipidome and fatty acid profiles were characterized by liquid chromatography-mass spectrometry (LC-MS), and gas chromatography-mass spectrometry (GC-MS), respectively. In addition, the antioxidant activity of their lipid extracts was assessed. The highest lipid contents were obtained for the two species grown indoors. LC-MS analysis identified 239 different polar lipid species, of which 220 were shared by all experimental groups. Candidate lipid biomarkers from both culture systems were proposed, including MGDG(34:2), MGDG(34:1) and PG(36:6). For both species, indoor conditions lead to lipid extracts rich in glycolipids and higher in oleic acid content. In contrast, outdoor conditions lead to higher proportions of phospholipids and betaine lipids and a higher relative content of eicosapentaenoic acid (EPA). The polar lipid profile of the two Nannochloropsis species differed primarily in the relative amounts of certain betaine lipids, mainly DGTS (which was increased in N. oceanica) and lysolipids (LPC, and LPE) (increased in N. limnetica), although the majority of lipids were observed in both species. The lipid extracts showed antioxidant activity (IC15) ranging from 30.4 +/- 1.8 to 45.7 +/- 1.6 mu mol Trolox g-1 of lipid extract. Overall, this study provides insight into the lipid metabolic adaptation of two Nannochloropsis species, providing the know-how to obtain a healthy polar lipid-rich biomass useful for novel applications in pharmaceutical, nutraceutical, or novel foods.pt_PT
dc.description.sponsorshipLISBOA-01-0145-FEDER-402-022125; POCI-01-0247-FEDER-035234
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.algal.2022.102718pt_PT
dc.identifier.issn2211-9264
dc.identifier.urihttp://hdl.handle.net/10400.1/18463
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationCentre for Environmental and Marine Studies
dc.relationCentre for Environmental and Marine Studies
dc.subjectMicroalgaept_PT
dc.subjectNannochloropsis oceanicapt_PT
dc.subjectNannochloropsis limneticapt_PT
dc.subjectLipidomicspt_PT
dc.subjectGrowth conditionspt_PT
dc.subjectPolar lipidspt_PT
dc.titleEffects of outdoor and indoor cultivation on the polar lipid composition and antioxidant activity of Nannochloropsis oceanica and Nannochloropsis limnetica: A lipidomics perspectivept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50017%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50017%2F2020/PT
oaire.citation.startPage102718pt_PT
oaire.citation.titleAlgal Researchpt_PT
oaire.citation.volume64pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNamePereira
person.familyNameVarela
person.givenNameHugo
person.givenNameJoão
person.identifier282781
person.identifier.ciencia-id331E-84FD-2F34
person.identifier.orcid0000-0002-1369-2099
person.identifier.orcid0000-0003-3101-693X
person.identifier.ridE-9360-2015
person.identifier.ridM-4223-2013
person.identifier.scopus-author-id54080259900
person.identifier.scopus-author-id55741416000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicationd339a9e7-bc32-49c4-ba48-a17343d40092
relation.isAuthorOfPublication.latestForDiscoveryd339a9e7-bc32-49c4-ba48-a17343d40092
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