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Batch operational stability of immobilized heterologous Rhizopus oryzae lipase during acidolysis of virgin olive oil with medium-chain fatty acids

2012Journal article Restricted access
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dc.contributor.authorNunes, Patrícia A.
dc.contributor.authorCabral, Paula Pires
dc.contributor.authorGuillen, M.
dc.contributor.authorValero, F.
dc.contributor.authorFerreira Dias, S.
dc.date.accessioned2016-02-03T16:15:08Z
dc.date.available2016-02-03T16:15:08Z
dc.date.issued2012
dc.description.abstractStructured triacylglycerols containing medium-chain fatty acids (M) at positions sn-1,3 and long-chain fatty acids (L) at the sn-2 position (MLM type), were obtained by acidolysis of virgin olive oil with caprylic or capric acid, in solvent-free media, at 40 degrees C, catalyzed by a heterologous Rhizopus oryzae lipase (r-ROL) immobilized in Eupergir (R) C or in Lewatit VP OC 1600. The biocatalyst immobilized in Eupergit was reused in consecutive 23-h batches with rehydration of the biocatalyst between batches. A first-order deactivation was observed (half-life time, t(1/2) = 39.0 h for caprylic; t(1/2) = 54.3 h for capric acid). During acidolysis with capric acid catalyzed by r-ROL immobilized in Lewatit VP OC 1600, without rehydration, a first-order deactivation was observed (t(1/2) = 49.1 h); with rehydration, a considerable increase in stability was observed (t(1/2) = 234 h; Sadana's series-type inactivation kinetics model). (C) 2012 Elsevier B.V. All rights reserved.
dc.identifier.doihttps://dx.doi.org/10.1016/j.bej.2012.06.004
dc.identifier.issn1369-703X
dc.identifier.otherAUT: PCA00486;
dc.identifier.urihttp://hdl.handle.net/10400.1/7585
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relation.isbasedonP-002-6X5
dc.titleBatch operational stability of immobilized heterologous Rhizopus oryzae lipase during acidolysis of virgin olive oil with medium-chain fatty acids
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage268
oaire.citation.startPage265
oaire.citation.titleBiochemical Engineering Journal
oaire.citation.volume67
person.familyNameNunes Cabrita
person.givenNamePatrícia
person.identifier.orcid0000-0002-7148-8950
person.identifier.scopus-author-id7006823084
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication4b0b5899-4a35-4405-acbe-a8f548457da1
relation.isAuthorOfPublication.latestForDiscovery4b0b5899-4a35-4405-acbe-a8f548457da1

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