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Ultra-/Small angle X-ray scattering (USAXS/SAXS) and static light scattering (SLS) modeling as a tool to determine structural changes and effect on growth in S. epidermidis

2022Journal article Restricted access
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dc.contributor.authorDuarte, Hugo
dc.contributor.authorGummel, Jeremie
dc.contributor.authorRobles, Eric
dc.contributor.authorBerti, Debora
dc.contributor.authorFratini, Emiliano
dc.date.accessioned2022-12-07T15:56:43Z
dc.date.available2023-07-29T00:30:16Z
dc.date.issued2022
dc.description.abstractUsually, to characterize bacterial cells' susceptibility to antimicrobials, basic microbiology techniques such as serial dilutions or disk assays are used. In this work, we present an approach focused on combining static light scattering (SLS) and ultra-/ small angle X-ray scattering (USAXS/SAXS). This approach was used to support microbiology techniques, with the aim of understanding the structural changes caused to bacteria when they are exposed to different stresses like pH, oxidation, and surfactants. Using USAXS/SAXS and SLS data, we developed a detailed multiscale model for a Gram-positive bacterium, S. epidermidis, and we extracted information regarding changes in the overall size and cell thickness induced by different stresses (i.e., pH and hydrogen peroxide). Increasing the concentration of hydrogen peroxide leads to a progressive reduction in cell wall thickness. Moreover, the concomitant use of pH and hydrogen peroxide provides evidence for a synergy in inhibiting the S. epidermidis growth. These promising results will be used as a starting base to further investigate more complex formulations and improve/refine the data modeling of bacteria in the small angle scattering regime.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1021/acsabm.2c00218pt_PT
dc.identifier.issn2576-6422
dc.identifier.urihttp://hdl.handle.net/10400.1/18601
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Chemical Societypt_PT
dc.relationWorld class Science and Innovation with Neutrons in Europe 2020 – SINE2020
dc.subjectSmall angle scatteringpt_PT
dc.subjectUSAXSSAXSpt_PT
dc.subjectStatic light scatteringpt_PT
dc.subjectBacteriapt_PT
dc.subjectAntimicrobialpt_PT
dc.subjectModelingpt_PT
dc.titleUltra-/Small angle X-ray scattering (USAXS/SAXS) and static light scattering (SLS) modeling as a tool to determine structural changes and effect on growth in S. epidermidispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleWorld class Science and Innovation with Neutrons in Europe 2020 – SINE2020
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/654000/EU
oaire.citation.endPage3712pt_PT
oaire.citation.issue8pt_PT
oaire.citation.startPage3703pt_PT
oaire.citation.titleACS Applied Bio Materialspt_PT
oaire.citation.volume5pt_PT
oaire.fundingStreamH2020
person.familyNameDuarte
person.givenNameHugo
person.identifier.ciencia-id8416-555E-B56F
person.identifier.orcid0000-0001-6461-3541
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationfb54c1ac-5e80-424a-acbe-989daacb3b14
relation.isAuthorOfPublication.latestForDiscoveryfb54c1ac-5e80-424a-acbe-989daacb3b14
relation.isProjectOfPublication464e1deb-bcee-4921-bade-da34080ff00c
relation.isProjectOfPublication.latestForDiscovery464e1deb-bcee-4921-bade-da34080ff00c

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