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Genetic diversity assessment of the almond (Prunus dulcis (Mill.) DA Webb) traditional germplasm of Algarve, Portugal, using molecular markers

dc.contributor.authorCabrita, L.
dc.contributor.authorApostolova, E.
dc.contributor.authorNeves, Alcinda
dc.contributor.authorMarreiros, A.
dc.contributor.authorLeitão, José
dc.date.accessioned2015-06-19T14:36:31Z
dc.date.available2015-06-19T14:36:31Z
dc.date.issued2014
dc.description.abstractIn this study, 123 almond (Prunus dulcis (Mill.) D. A. Webb) trees identified among traditional orchards in the Algarve region and 53 trees of the local field collection managed by the regional office of the Portuguese Ministry of Agriculture (DRAALG) were assessed using isozyme, inter- single sequence repeat and simple sequence repeat or microsatellite techniques for the evaluation of genetic diversity and genetic relatedness and identification of new accessions for the field collection. The isozyme analysis allowed the distribution of the 176 plants into 13 different classes of enzyme similarity, while the use of DNA markers increased the distribution of the analysed trees among 140 discriminating DNA patterns. Multiple cases of homonymy and synonymy were identified in the local germplasm. Some traditional varieties, such as Lourencinha, appeared to be relatively homogeneous, while other local denominations, e.g. Galamba, included diverse genotypes. Of the 13 commercial varieties analysed in this study, 11 assembled in one major cluster clearly differentiated from the majority of the local genotypes. These results reinforced the perception that the Algarve traditional germplasm constitutes an important repository of genetic diversity, eventually carrying alleles of high agricultural interest such as the recently identified Phomopsis resistance in the traditional variety Barrinho Grado.
dc.identifier.doihttps://dx.doi.org/10.1017/S1479262114000471
dc.identifier.issn1479-2621
dc.identifier.issn1479-263X
dc.identifier.otherAUT: JLE00446; MAN00264;
dc.identifier.urihttp://hdl.handle.net/10400.1/6542
dc.language.isoeng
dc.peerreviewedyes
dc.publisherCambridge University Press
dc.relation.isbasedonP-009-Q0Q
dc.titleGenetic diversity assessment of the almond (Prunus dulcis (Mill.) DA Webb) traditional germplasm of Algarve, Portugal, using molecular markers
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPageS167
oaire.citation.startPageS164
oaire.citation.titlePlant Genetic Resources: Characterization and Utilization
oaire.citation.volume12
person.familyNameLeitão
person.givenNameJosé
person.identifier258398
person.identifier.ciencia-id7A17-3E0A-73AD
person.identifier.orcid0000-0003-4278-0415
person.identifier.ridAAH-3958-2020
person.identifier.scopus-author-id7005119796
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication0f990d1c-2913-4f8a-8fb6-f8b02721247f
relation.isAuthorOfPublication.latestForDiscovery0f990d1c-2913-4f8a-8fb6-f8b02721247f

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