Repository logo
 
Publication

Determination of tissue and plasma concentrations of PTHrP in fish: development and validation of a radioimmunoassay using a teleost 1–34 N-terminal peptide

2003Journal article Restricted access
Simple item page
dc.contributor.authorRotllant, J.
dc.contributor.authorWorthington, G. P.
dc.contributor.authorFuentes, J.
dc.contributor.authorGuerreiro, P. M.
dc.contributor.authorTeitsma, C. A.
dc.contributor.authorIngleton, P. M.
dc.contributor.authorBalment, R.
dc.contributor.authorCanario, Adelino V. M.
dc.contributor.authorPower, Deborah
dc.date.accessioned2014-05-12T11:01:31Z
dc.date.available2014-05-12T11:01:31Z
dc.date.issued2003
dc.date.updated2014-05-09T09:32:46Z
dc.description.abstractA specific and sensitive radioimmunoassay (RIA) for the N-terminus of sea bream (Sparus auratus) and flounder (Platichthys flesus) parathyroid hormone-related protein (PTHrP) was developed. A (1–34) amino-terminal sequence of flounder PTHrP was synthesized commercially and used as the antigen to generate specific antiserum. The same sequence with an added tyrosine (1– 35Tyr) was used for iodination. Human (1–34) parathyroid hormone (PTH), human (1–34) PTHrP, and rat (1–34) PTHrP did not cross-react with the antiserum or displace the teleost peptide. Measurement of PTHrP in fish plasma was only possible after denaturing by heat treatment due to endogenous plasma binding activity. The minimum detectable concentration of (1–34) PTHrP in the assay was 2.5 pg/tube. The level of immunoreactive (1–34) PTHrP in plasma was 5.2 0.44 ng/ml (mean SEM, n ¼ 20) for flounder and 2.5 0.29 ng/ml (n ¼ 64) for sea bream. Dilution curves of denatured fish plasma were parallel to the assay standard curve, indicating that the activity in the samples was indistinguishable immunologically from (1–34) PTHrP. Immunoreactivity was present, in order of abundance, in extracts of pituitary, oesophagus, kidney, head kidney, gills, intestine, skin, muscle, and liver. The pituitary gland and oesophagus contained the most abundant levels of PTHrP, 37.7 6.1 ng/g wet tissue and 2.3 0.7 ng/g wet tissue, respectively. The results suggest that in fish PTHrP may act in a paracrine and/or autocrine manner but may also be a classical hormone with the pituitary gland as a potential major source of the protein.por
dc.identifier.citationRotllant, J; Worthington, G.P; Fuentes, J; Guerreiro, P.M; Teitsma,C.A; Ingleton, P.M; Balment, R.J; Canario, A.V.M; Power, D.M. Determination of tissue and plasma concentrations of PTHrP in fish: development and validation of a radioimmunoassay using a teleost 1–34 N-terminal peptide, General and Comparative Endocrinology, 133, 1, 146-153, 2003.por
dc.identifier.doihttp://dx.doi.org/10.1016/S0016-6480(03)00166-7
dc.identifier.issn0016-6480
dc.identifier.otherAUT: ACA00258; DPO00386;
dc.identifier.urihttp://hdl.handle.net/10400.1/3862
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherAcademic Presspor
dc.subjectParathyroid hormone-related protein (PTHrP)por
dc.subjectTissue distributionpor
dc.subjectRadioimmunoassaypor
dc.subjectFlounder (Platichthys flesus)por
dc.subjectSea bream (Sparus auratus)por
dc.titleDetermination of tissue and plasma concentrations of PTHrP in fish: development and validation of a radioimmunoassay using a teleost 1–34 N-terminal peptidepor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage153por
oaire.citation.issue1por
oaire.citation.startPage146por
oaire.citation.titleGeneral and Comparative Endocrinologypor
oaire.citation.volume133por
person.familyNameFuentes
person.familyNameGuerreiro da Costa Guerreiro
person.familyNameCanario
person.familyNamePower
person.givenNameJuan
person.givenNamePedro Miguel
person.givenNameAdelino
person.givenNameDeborah Mary
person.identifier392998
person.identifierA-2539-2009
person.identifier143624
person.identifier.ciencia-id421B-E196-2C33
person.identifier.ciencia-id5C13-965D-3148
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0003-0430-8734
person.identifier.orcid0000-0001-5371-7919
person.identifier.orcid0000-0002-6244-6468
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id7201832526
person.identifier.scopus-author-id56568523700
person.identifier.scopus-author-id7101806760
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationd354b937-b18c-4ca5-a843-04a04269d60f
relation.isAuthorOfPublicationd0a8a148-98e7-4899-8fb0-0b8afa91e4a4
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscoveryd354b937-b18c-4ca5-a843-04a04269d60f

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Determination of tissue and plasma concentrations of PTHrP in fish Development and validation of a radioimmunoassay using a teleost 1–34 N-terminal peptide.pdf
Size:
195.39 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.61 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)