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Antemortem versus postmortem methods for detection of betanodavirus in Senegalese sole (Solea senegalensis)

2008Journal article Open access
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dc.contributor.authorOlveira, J. G.
dc.contributor.authorSoares, F.
dc.contributor.authorEngrola, Sofia
dc.contributor.authorDopazo, C. P.
dc.contributor.authorBandín, I.
dc.date.accessioned2014-07-04T09:06:35Z
dc.date.available2014-07-04T09:06:35Z
dc.date.issued2008
dc.date.updated2014-07-03T11:26:07Z
dc.description.abstractThe suitability of nested reverse transcription polymerase chain reaction (nRT-PCR) to detect betanodavirus in blood samples from naturally infected Senegalese sole (Solea senegalensis) was evaluated in comparison with other diagnostic methods. Results indicated that histologic examination of brain lesions could be regarded as the most consistent indicator of nodavirus infection in this species. The nRT-PCR showed low to moderate levels of detection; the best values were obtained in brain samples followed by blood samples. Inoculation of SSN-1 and SAF-1 cells with fish samples did not cause cytopathic effect, although virus was detected by reverse transcription polymerase chain reaction in approximately 25% of the SSN-1 inoculated wells. The efficiency of detection of the viral genome was dramatically increased by the use of nRTPCR, reaching 90.6% of positives in brain samples and 84.4% in blood samples. The sensitivity and the negative predictive value of nRT-PCR in blood samples were slightly lower than those obtained using brain samples. Nevertheless, it is suggested that the advantage of being able to perform diagnosis on live fish adequately counterbalances the slightly lower sensitivity of nRT-PCR on blood samples. This technique is proposed as a useful tool, not only for the selection of nodavirus-free breeders but also to check the fish status during ongrowing.por
dc.identifier.citationOlveira, J.G.; Soares, F.; Engrola, S.; Dopazo, C.P.; Bandín, I. Antemortem versus postmortem methods for detection of betanodavirus in Senegalese sole (Solea senegalensis), Journal of Veterinary Diagnostic Investigation, 20, 2, 215-219, 2008.por
dc.identifier.doihttp://dx.doi.org/10.1177/104063870802000212
dc.identifier.issn1040-6387
dc.identifier.urihttp://hdl.handle.net/10400.1/4648
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherAmerican Association of Veterinary Laboratory Diagnosticianspor
dc.relation.publisherversionhttp://vdi.sagepub.com/content/20/2/215por
dc.subjectBetanodaviruspor
dc.subjectBloodpor
dc.subjectNested reverse transcription polymerase chain reactionpor
dc.titleAntemortem versus postmortem methods for detection of betanodavirus in Senegalese sole (Solea senegalensis)por
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage219por
oaire.citation.issue2por
oaire.citation.startPage215por
oaire.citation.titleJournal of Veterinary Diagnostic Investigationpor
oaire.citation.volume20por
person.familyNameEngrola
person.givenNameSofia
person.identifier237846
person.identifier.ciencia-idA610-29E7-9B83
person.identifier.orcid0000-0002-5244-5541
person.identifier.ridA-2485-2012
person.identifier.scopus-author-id8669620600
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication51be5a20-df9c-4a90-85eb-51d779642caa
relation.isAuthorOfPublication.latestForDiscovery51be5a20-df9c-4a90-85eb-51d779642caa

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