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Insights on the association between somatic aneuploidy and ostreid herpesvirus 1 detection in the oysters Crassostrea gigas, C. angulata and their F1 hybrids

2016-05Journal article Restricted access
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dc.contributor.authorBatista, Frederico
dc.contributor.authorLopez-Sanmartin, Monserrat
dc.contributor.authorBoudry, Pierre
dc.contributor.authorNavas, Jose I.
dc.contributor.authorRuano, Francisco
dc.contributor.authorRenault, Tristan
dc.contributor.authorFonseca, Vera G.
dc.contributor.authorLeitao, Alexandra
dc.date.accessioned2017-04-07T15:56:43Z
dc.date.available2017-04-07T15:56:43Z
dc.date.issued2016-05
dc.description.abstractCytogenetic abnormalities associated with viral infections, including from viruses of the Herpesvirales order, have been reported in vertebrate species. Ostreid herpesvirus 1 (OsHV-1) has been detected worldwide during mortality outbreaks of the Pacific oyster Crassostrea gigas. On the other hand, a high proportion of aneuploid cells in somatic tissues have been observed in C. gigas. In this study, we analysed the putative association between aneuploidy levels and the detection of OsHV-1 in gills of C. gigas, the Portuguese oyster C. angulata and their F1 hybrids cultured in Ria Formosa (Portugal). OsHV-1 was detected by PCR in 5.4% of the total of oysters analysed (n = 111) namely in 11.1%, 8.0% and 1.7% of C. gigas, C. angulata and F1 hybrid respectively. Sequencing analysis of a viral fragment amplified with the C2/C6 primer pair revealed a high similarity with the OsHV-1 reference type. Moreover, in situ hybridization confirmed the presence of OsHV-1 in gill tissue. Oysters where OsHV-1 was detected had a significantly higher mean percentage of aneuploid cells (25%) than the ones where the virus was not detected (18%). However, the overall low percentage of positive samples contrasted with the high mean percentage of aneuploidy observed, with 50% of the oysters analysed showing a percentage of aneuploid cells between 20% and 30%. We hypothesize that somatic aneuploidy may adversely affect oysters making them more prone to OsHV-1 infection, but the virus is unlikely to be the cause of somatic aneuploidy.
dc.description.sponsorship(PLUDAMOR, QLK5-CT-2000-60036)
dc.identifier.doi10.1111/are.12613
dc.identifier.issn1355-557X
dc.identifier.urihttp://hdl.handle.net/10400.1/9505
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley
dc.relation.isbasedonWOS:000373793200015
dc.titleInsights on the association between somatic aneuploidy and ostreid herpesvirus 1 detection in the oysters Crassostrea gigas, C. angulata and their F1 hybrids
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F8972%2F2002/PT
oaire.citation.endPage1536
oaire.citation.issue5
oaire.citation.startPage1530
oaire.citation.titleAquaculture Research
oaire.citation.volume47
oaire.fundingStreamSFRH
person.familyNameBatista
person.givenNameFrederico
person.identifier.ciencia-idCB1B-7DBC-6303
person.identifier.orcid0000-0002-2848-3011
person.identifier.ridF-5651-2010
person.identifier.scopus-author-id7003808684
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublicationd564e68d-a75e-458f-add4-bdb0739cde8d
relation.isAuthorOfPublication.latestForDiscoveryd564e68d-a75e-458f-add4-bdb0739cde8d
relation.isProjectOfPublication089d3227-6c1b-488f-945c-2523f93d58c5
relation.isProjectOfPublication.latestForDiscovery089d3227-6c1b-488f-945c-2523f93d58c5

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