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Exosome delivery to the testes for dmrt1 suppression: a powerful tool for sex-determining gene studies

2023Journal article Open access
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dc.contributor.authorZhu, Tengfei
dc.contributor.authorKong, Ming
dc.contributor.authorYu, Yingying
dc.contributor.authorSchartl, Manfred
dc.contributor.authorPower, Deborah
dc.contributor.authorLi, Chen
dc.contributor.authorMa, Wenxiu
dc.contributor.authorSun, Yanxu
dc.contributor.authorLi, Shuo
dc.contributor.authorYue, Bowen
dc.contributor.authorLi, Weijing
dc.contributor.authorShao, Changwei
dc.date.accessioned2023-12-11T11:35:33Z
dc.date.available2023-12-11T11:35:33Z
dc.date.issued2023
dc.description.abstractExosomes are endosome-derived extracellular vesicles about 100 nm in diameter. They are emerging as prom ising delivery platforms due to their advantages in biocompatibility and engineerability. However, research into and applications for engineered exosomes are still limited to a few areas of medicine in mammals. Here, we expanded the scope of their applications to sex-determining gene studies in early vertebrates. An integrated strategy for constructing the exosome-based delivery system was developed for efficient regulation of dmrt1, which is one of the most widely used sex-determining genes in metazoans. By combining classical methods in molecular biology and the latest technology in bioinformatics, isomiR-124a was identified as a dmrt1 inhibitor and was loaded into exosomes and a testis-targeting peptide was used to modify exosomal surface for efficient delivery. Results showed that isomiR-124a was efficiently delivered to the testes by engineered exosomes and revealed that dmrt1 played important roles in maintaining the regular structure and function of testis in juvenile fish. This is the first de novo development of an exosome-based delivery system applied in the study of sex determining gene, which indicates an attractive prospect for the future applications of engineered exosomes in exploring more extensive biological conundrums.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.jconrel.2023.09.027pt_PT
dc.identifier.issn0168-3659
dc.identifier.urihttp://hdl.handle.net/10400.1/20205
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEngineered exosomept_PT
dc.subjectDelivery systempt_PT
dc.subjectTissue targetingpt_PT
dc.subjectdmrt1pt_PT
dc.subjectSex-determining genept_PT
dc.subjectMicroRNApt_PT
dc.titleExosome delivery to the testes for dmrt1 suppression: a powerful tool for sex-determining gene studiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage289pt_PT
oaire.citation.startPage275pt_PT
oaire.citation.titleJournal of Controlled Releasept_PT
oaire.citation.volume363pt_PT
person.familyNamePower
person.givenNameDeborah Mary
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0003-1366-0246
person.identifier.scopus-author-id7101806760
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscoveryc68f5ffb-63f6-4c70-8957-29e464fb59c0

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