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Matrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulation

2009Artigo científico Acesso aberto
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dc.contributor.authorRoberto, Vania Palma
dc.contributor.authorCavaco, S.
dc.contributor.authorS B Viegas, Carla
dc.contributor.authorSimes, D
dc.contributor.authorOrtiz-Delgado, J. B.
dc.contributor.authorSarasquete, C.
dc.contributor.authorGavaia, Paulo J.
dc.contributor.authorCancela, Leonor
dc.date.accessioned2014-06-04T13:28:35Z
dc.date.available2014-06-04T13:28:35Z
dc.date.issued2009
dc.date.updated2014-05-30T10:04:41Z
dc.description.abstractMatrix Gla protein (Mgp) is a secreted vitamin K-dependent extracellular matrix protein and a physiological inhibitor of calcification whose gene structure, amino acid sequence and tissue distribution have been conserved throughout evolution. In the present work, the turbot (Scophthalmus maximus) mgp cDNA was cloned and the sequence of the deduced protein compared to that of other vertebrates. As expected, it was closer to teleosts than to other vertebrate groups but there was a strict conservation of amino-acids thought to be important for protein function. Analysis of mgp gene expression indicated branchial arches as the site with higher levels of expression, followed by heart, vertebra and kidney. These results were confirmed by in situ hybridization with a strong mgp expression in branchial arch chondrocytes. Mgp was found to accumulate in gills where it appeared to be restricted to chondrocytes from branchial filaments, while in vertebrae it was localized in vertebral end plates, in growth zones, in vertebral arches and spines and in notochord cells. In the soft tissues analysed, Mgp was mainly detected in kidney and heart, consistent with previous data and providing further evidence for a role of Mgp as a calcification inhibitor and a modulator of the mineralization process. Our studies provide evidence that turbot, an important new species for aquaculture, is also a useful model to study function and expression of Mgp.por
dc.identifier.citationRoberto, V.P.; Cavaco, S.; Viegas, C.S.B.; Simes, D.C.; Ortiz-Delgado, J.-B.; Sarasquete, M.C.; Gavaia, P.J.; Cancela, M.L. Matrix Gla protein in turbot (Scophthalmus maximus): Gene expression analysis and identification of sites of protein accumulation, Aquaculture, 294, 3-4, 202-211, 2009.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.aquaculture.2009.06.020
dc.identifier.issn0044-8486
dc.identifier.otherAUT: DSI00813; LCA00739;
dc.identifier.urihttp://hdl.handle.net/10400.1/4184
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0044848609005353por
dc.subjectTurbot Mgppor
dc.subjectGene expressionpor
dc.subjectSoft tissue accumulationpor
dc.subjectHeartpor
dc.subjectVertebraepor
dc.subjectBranchial archespor
dc.titleMatrix gla protein in turbot (Scophthalmus maximus): gene expression analysis and identification of sites of protein accumulationpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage211por
oaire.citation.issue3-4por
oaire.citation.startPage202por
oaire.citation.titleAquaculturepor
oaire.citation.volume294por
person.familyNameRoberto
person.familyNameViegas
person.familyNameSimes
person.familyNameGavaia
person.familyNameCancela
person.givenNameVânia
person.givenNameCarla
person.givenNameDina
person.givenNamePaulo
person.givenNameM. Leonor
person.identifier1230353
person.identifier.ciencia-id681E-40B7-331F
person.identifier.ciencia-idC414-F596-EEC6
person.identifier.ciencia-idB619-FC16-D007
person.identifier.orcid0000-0002-8134-4045
person.identifier.orcid0000-0002-5765-3665
person.identifier.orcid0000-0002-5145-4753
person.identifier.orcid0000-0002-9582-1957
person.identifier.orcid0000-0003-3114-6662
person.identifier.ridN-6695-2014
person.identifier.ridN-2789-2014
person.identifier.ridA-6470-2011
person.identifier.scopus-author-id27467657800
person.identifier.scopus-author-id8656310300
person.identifier.scopus-author-id7201884723
person.identifier.scopus-author-id6507104377
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationc730a868-0e9f-43e4-a354-10130b30b764
relation.isAuthorOfPublication33984b3c-ffac-477a-896a-ddbf7aced4e3
relation.isAuthorOfPublication31bac96d-1d01-4b8f-8f2b-9da4be31ea41
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relation.isAuthorOfPublicationb9bbfe32-3dfe-4131-ad14-a4394008447f
relation.isAuthorOfPublication.latestForDiscovery33984b3c-ffac-477a-896a-ddbf7aced4e3

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