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Expressed sequence tags from heat-shocked seagrass Zostera noltii (Hornemann) from its southern distribution range

dc.contributor.authorMassa, S. I.
dc.contributor.authorPearson, G. A.
dc.contributor.authorAires, Tânia
dc.contributor.authorKube, M.
dc.contributor.authorOlsen, J. L.
dc.contributor.authorReinhardt, Richard
dc.contributor.authorSerrão, Ester
dc.contributor.authorARNAUD-HAOND, Sophie
dc.date.accessioned2014-05-27T11:37:18Z
dc.date.available2014-05-27T11:37:18Z
dc.date.issued2011
dc.date.updated2014-05-21T11:42:39Z
dc.description.abstractPredicted global climate change threatens the distributional ranges of species worldwide. We identified genes expressed in the intertidal seagrass Zostera noltii during recovery from a simulated low tide heat-shock exposure. Five Expressed Sequence Tag (EST) libraries were compared, corresponding to four recovery times following sub-lethal temperature stress, and a non-stressed control. We sequenced and analyzed 7009 sequence reads from 30 min, 2 h, 4 h and 24 h after the beginning of the heat-shock (AHS), and 1585 from the control library, for a total of 8594 sequence reads. Among 51 Tentative UniGenes (TUGs) exhibiting significantly different expression between libraries, 19 (37.3%) were identified as ‘molecular chaperones’ and were over-expressed following heat-shock, while 12 (23.5%) were ‘photosynthesis TUGs’ generally under-expressed in heat-shocked plants. A time course analysis of expression showed a rapid increase in expression of the molecular chaperone class, most of which were heat-shock proteins; which increased from 2 sequence reads in the control library to almost 230 in the 30 min AHS library, followed by a slow decrease during further recovery. In contrast, ‘photosynthesis TUGs’ were under-expressed 30 min AHS compared with the control library, and declined progressively with recovery time in the stress libraries, with a total of 29 sequence reads 24 h AHS, compared with 125 in the control. A total of 4734 TUGs were screened for EST-Single Sequence Repeats (EST-SSRs) and 86 microsatellites were identified.► Response to heat stress is very fast but gene expression returns to normal after 24 h. ► Photosynthesis-related genes were under-expressed after heat-shock. ► Heat-shock caused a quick rise in heat shock proteins and molecular chaperone expression.por
dc.identifier.citationMassa, S.I.; Pearson, G.A.; Aires, T.; Kube, M.; Olsen, J.L.; Reinhardt, R.; Serrão, E.A.; Arnaud-Haond, S. Expressed sequence tags from heat-shocked seagrass Zostera noltii (Hornemann) from its southern distribution range, Marine Genomics, 4, 3, 181-188, 2011.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.margen.2011.04.003
dc.identifier.issn1874-7787
dc.identifier.otherAUT: ESE00527;
dc.identifier.urihttp://hdl.handle.net/10400.1/4092
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectEST librarypor
dc.subjectClimate changepor
dc.subjectAbiotic stress responsepor
dc.subjectHigh temperature stresspor
dc.titleExpressed sequence tags from heat-shocked seagrass Zostera noltii (Hornemann) from its southern distribution rangepor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage188por
oaire.citation.issue3por
oaire.citation.startPage181por
oaire.citation.titleMarine Genomicspor
oaire.citation.volume4por
person.familyNamePearson
person.familyNameAires
person.familyNameSerrao
person.familyNameARNAUD-HAOND
person.givenNameGareth Anthony
person.givenNameTania
person.givenNameEster A.
person.givenNameSophie
person.identifier113536
person.identifier282868
person.identifierC-6686-2012
person.identifier.ciencia-id3315-9919-1A52
person.identifier.ciencia-id4318-9E2C-32B9
person.identifier.ciencia-id5B13-B26E-B1EC
person.identifier.ciencia-id5A15-FF67-4075
person.identifier.orcid0000-0002-0768-464X
person.identifier.orcid0000-0002-1964-6819
person.identifier.orcid0000-0003-1316-658X
person.identifier.orcid0000-0001-5814-8452
person.identifier.ridJ-3911-2013
person.identifier.ridM-8306-2013
person.identifier.scopus-author-id55916875600
person.identifier.scopus-author-id7004093604
person.identifier.scopus-author-id6602532118
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublication.latestForDiscovery0a10c448-c7f5-4c3b-9488-3917c707e35e

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