Publication
Expressed sequence tags from heat-shocked seagrass Zostera noltii (Hornemann) from its southern distribution range
dc.contributor.author | Massa, S. I. | |
dc.contributor.author | Pearson, G. A. | |
dc.contributor.author | Aires, Tânia | |
dc.contributor.author | Kube, M. | |
dc.contributor.author | Olsen, J. L. | |
dc.contributor.author | Reinhardt, Richard | |
dc.contributor.author | Serrão, Ester | |
dc.contributor.author | ARNAUD-HAOND, Sophie | |
dc.date.accessioned | 2014-05-27T11:37:18Z | |
dc.date.available | 2014-05-27T11:37:18Z | |
dc.date.issued | 2011 | |
dc.date.updated | 2014-05-21T11:42:39Z | |
dc.description.abstract | Predicted global climate change threatens the distributional ranges of species worldwide. We identified genes expressed in the intertidal seagrass Zostera noltii during recovery from a simulated low tide heat-shock exposure. Five Expressed Sequence Tag (EST) libraries were compared, corresponding to four recovery times following sub-lethal temperature stress, and a non-stressed control. We sequenced and analyzed 7009 sequence reads from 30 min, 2 h, 4 h and 24 h after the beginning of the heat-shock (AHS), and 1585 from the control library, for a total of 8594 sequence reads. Among 51 Tentative UniGenes (TUGs) exhibiting significantly different expression between libraries, 19 (37.3%) were identified as ‘molecular chaperones’ and were over-expressed following heat-shock, while 12 (23.5%) were ‘photosynthesis TUGs’ generally under-expressed in heat-shocked plants. A time course analysis of expression showed a rapid increase in expression of the molecular chaperone class, most of which were heat-shock proteins; which increased from 2 sequence reads in the control library to almost 230 in the 30 min AHS library, followed by a slow decrease during further recovery. In contrast, ‘photosynthesis TUGs’ were under-expressed 30 min AHS compared with the control library, and declined progressively with recovery time in the stress libraries, with a total of 29 sequence reads 24 h AHS, compared with 125 in the control. A total of 4734 TUGs were screened for EST-Single Sequence Repeats (EST-SSRs) and 86 microsatellites were identified.► Response to heat stress is very fast but gene expression returns to normal after 24 h. ► Photosynthesis-related genes were under-expressed after heat-shock. ► Heat-shock caused a quick rise in heat shock proteins and molecular chaperone expression. | por |
dc.identifier.citation | Massa, S.I.; Pearson, G.A.; Aires, T.; Kube, M.; Olsen, J.L.; Reinhardt, R.; Serrão, E.A.; Arnaud-Haond, S. Expressed sequence tags from heat-shocked seagrass Zostera noltii (Hornemann) from its southern distribution range, Marine Genomics, 4, 3, 181-188, 2011. | por |
dc.identifier.doi | http://dx.doi.org/10.1016/j.margen.2011.04.003 | |
dc.identifier.issn | 1874-7787 | |
dc.identifier.other | AUT: ESE00527; | |
dc.identifier.uri | http://hdl.handle.net/10400.1/4092 | |
dc.language.iso | eng | por |
dc.peerreviewed | yes | por |
dc.publisher | Elsevier | por |
dc.subject | EST library | por |
dc.subject | Climate change | por |
dc.subject | Abiotic stress response | por |
dc.subject | High temperature stress | por |
dc.title | Expressed sequence tags from heat-shocked seagrass Zostera noltii (Hornemann) from its southern distribution range | por |
dc.type | journal article | |
dspace.entity.type | Publication | |
oaire.citation.endPage | 188 | por |
oaire.citation.issue | 3 | por |
oaire.citation.startPage | 181 | por |
oaire.citation.title | Marine Genomics | por |
oaire.citation.volume | 4 | por |
person.familyName | Pearson | |
person.familyName | Aires | |
person.familyName | Serrao | |
person.familyName | ARNAUD-HAOND | |
person.givenName | Gareth Anthony | |
person.givenName | Tania | |
person.givenName | Ester A. | |
person.givenName | Sophie | |
person.identifier | 113536 | |
person.identifier | 282868 | |
person.identifier | C-6686-2012 | |
person.identifier.ciencia-id | 3315-9919-1A52 | |
person.identifier.ciencia-id | 4318-9E2C-32B9 | |
person.identifier.ciencia-id | 5B13-B26E-B1EC | |
person.identifier.ciencia-id | 5A15-FF67-4075 | |
person.identifier.orcid | 0000-0002-0768-464X | |
person.identifier.orcid | 0000-0002-1964-6819 | |
person.identifier.orcid | 0000-0003-1316-658X | |
person.identifier.orcid | 0000-0001-5814-8452 | |
person.identifier.rid | J-3911-2013 | |
person.identifier.rid | M-8306-2013 | |
person.identifier.scopus-author-id | 55916875600 | |
person.identifier.scopus-author-id | 7004093604 | |
person.identifier.scopus-author-id | 6602532118 | |
rcaap.rights | restrictedAccess | por |
rcaap.type | article | por |
relation.isAuthorOfPublication | 0a10c448-c7f5-4c3b-9488-3917c707e35e | |
relation.isAuthorOfPublication | bb3e6904-8ea3-43e6-8c43-4979daafec9b | |
relation.isAuthorOfPublication | 45ccfe90-155c-4d6f-9e86-8f0fd064005f | |
relation.isAuthorOfPublication | 2b10c79a-8ca9-4449-ba02-0c240f00d921 | |
relation.isAuthorOfPublication.latestForDiscovery | 0a10c448-c7f5-4c3b-9488-3917c707e35e |
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