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Extracellular electrophysiological based sensor to monitor cancer cells cooperative migration and cell-cell connections

2019-12Journal article Restricted access
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dc.contributor.authorAsgarifar, Sanaz
dc.contributor.authorMestre, Ana L. G.
dc.contributor.authorFélix, Rute
dc.contributor.authorInacio, Pedro M. C.
dc.contributor.authorLurdes S. Cristiano, M.
dc.contributor.authorMedeiros, Maria C. R.
dc.contributor.authorAraújo, Inês
dc.contributor.authorPower, Deborah
dc.contributor.authorGomes, Henrique L.
dc.date.accessioned2020-07-24T10:50:52Z
dc.date.available2021-12-01T01:30:15Z
dc.date.issued2019-12
dc.description.abstractHerein, we describe an electrophysiological based sensor that reproducibly monitors and quantifies in real-time collective migration and the formation of cell-cell junctions by C6 glioma cells seeded on top of electrodes. The signal amplitude and frequency generated by the migrating cells changed over time and these parameters were used to accurately calculate the migration speed. Electrophysiological measurements could also distinguish individual from collective cell migration. The migration of densely packed cells generated strong signals, while dispersed cells showed weak bioelectrical activity. We propose this electrophysiological technique as a cell-based biosensor to gain insight into the mechanisms of cooperative migration of cancer cells. Possible applications include screening for anti-migratory compounds, which may lead to the development of novel strategies for antineoplastic chemotherapy.
dc.description.sponsorshipPortuguese Foundation for Science and Technology (FCT)Portuguese Foundation for Science and Technology [PTDC/EEI-AUT/5442/2014, UID/EEA/50008/2019, UID/BIM/04773/2019, UID/Multi/04326/2019]
dc.description.sponsorshipUniversidade do Algarve
dc.identifier.doi10.1016/j.bios.2019.111708
dc.identifier.issn0956-5663
dc.identifier.issn1873-4235
dc.identifier.urihttp://hdl.handle.net/10400.1/14164
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier Advanced Technology
dc.subjectIon channels
dc.subjectOscillations
dc.subjectBiosensor
dc.subjectArrays
dc.subjectWaves
dc.titleExtracellular electrophysiological based sensor to monitor cancer cells cooperative migration and cell-cell connections
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPageUNSP 111708
oaire.citation.titleBiosensors and Bioelectronics
oaire.citation.volume145
person.familyNameAsgarifar
person.familyNameFélix
person.familyNameCristiano
person.familyNamePombinho de Araújo
person.familyNamePower
person.givenNameSanaz
person.givenNameRute
person.givenNameMaria de Lurdes
person.givenNameInês Maria
person.givenNameDeborah Mary
person.identifierhttps://scholar.google.com/citations?hl=en&user=LFpifG0AAAAJ
person.identifierF-4703-2012
person.identifier.ciencia-idE911-30E7-9D0C
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person.identifier.ciencia-idD11F-D4CA-2947
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0002-8724-0044
person.identifier.orcid0000-0002-4144-3452
person.identifier.orcid0000-0002-9447-2855
person.identifier.orcid0000-0002-2438-0111
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridM-6164-2013
person.identifier.ridG-2345-2012
person.identifier.scopus-author-id24823836800
person.identifier.scopus-author-id56006773000
person.identifier.scopus-author-id9238724800
person.identifier.scopus-author-id56271084100
person.identifier.scopus-author-id7101806760
rcaap.rightsopenAccesspt_PT
rcaap.typearticle
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