Repository logo
 
Publication

Role of the melanocortin system in zebrafish skin physiology

2022Journal article Open access
Simple item page
dc.contributor.authorLeal, E.
dc.contributor.authorAngotzi, A. R.
dc.contributor.authorGregorio, S. F.
dc.contributor.authorOrtiz-Delgado, J. B.
dc.contributor.authorRotllant, J.
dc.contributor.authorFuentes, Juan
dc.contributor.authorTafalla, C.
dc.contributor.authorCerdá-Reverter, J. M.
dc.date.accessioned2023-01-17T12:06:57Z
dc.date.available2023-01-17T12:06:57Z
dc.date.issued2022
dc.description.abstractThe agouti-signaling protein (ASIP) acts as both a competitive antagonist and inverse agonist of melanocortin receptors which regulate dorsal-ventral pigmentation patterns in fish. However, the potential role of ASIP in the regulation of additional physiological pathways in the skin is unknown. The skin plays a crucial role in the immune function, acting as a physical limitation against infestation and also as a chemical barrier due to its ability to synthesize and secrete mucus and many immune effector proteins. In this study, the putative role of ASIP in regulating the immune system of skin has been explored using a transgenic zebrafish model over -expressing the asip1 gene (ASIPzf). Initially, the structural changes in skin induced by asip1 overexpression were studied, revealing that the ventral skin of ASIPzf was thinner than that of wild type (WT) animals. A moderate hypertrophy of mucous cells was also found in ASIPzf. Histochemical studies showed that transgenic animals appear to compensate for the lower number of cell layers by modifying the mucus composition and increasing lectin affinity and mucin content in order to maintain or improve protection against microorganism adhesion. ASIPzf also exhibit higher protein concentration under crowding conditions suggesting an increased mucus production under stressful conditions. Exposure to bacterial lipopolysaccharide (LPS) showed that ASIPzf exhibit a faster pro-inflammatory response and increased mucin expression yet severe skin injures and a slight increase in mortality was observed. Electrophysiological measurements show that the ASIP1 genotype exhibits reduced epithelial resistance, an indicator of reduced tissue integrity and barrier function. Overall, not only are ASIP1 animals more prone to infiltration and subsequent infections due to reduced skin epithelial integrity, but also display an increased inflammatory response that can lead to increased skin sensitivity to external infections.pt_PT
dc.description.sponsorshipRTI2018-093909-B-100
dc.description.sponsorshipAGL2017-89648P
dc.description.sponsorshipLA/P/0101/2020
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.fsi.2022.09.036pt_PT
dc.identifier.eissn1095-9947
dc.identifier.urihttp://hdl.handle.net/10400.1/18836
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAcademic Press LTD - Elsevierpt_PT
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAgouti -signalling proteinpt_PT
dc.subjectMelanocyte-stimulating hormone (MSH)pt_PT
dc.subjectLPSpt_PT
dc.subjectCytokinept_PT
dc.subjectMucuspt_PT
dc.subjectImmune responsept_PT
dc.subjectStresspt_PT
dc.titleRole of the melanocortin system in zebrafish skin physiologypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.citation.endPage601pt_PT
oaire.citation.startPage591pt_PT
oaire.citation.titleFish & Shellfish Immunologypt_PT
oaire.citation.volume130pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameGregorio
person.familyNameFuentes
person.givenNameSilvia
person.givenNameJuan
person.identifier1579837
person.identifier392998
person.identifier.ciencia-idBF14-020B-4C39
person.identifier.ciencia-id421B-E196-2C33
person.identifier.orcid0000-0002-6648-5122
person.identifier.orcid0000-0003-0430-8734
person.identifier.ridG-1618-2018
person.identifier.scopus-author-id7201832526
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication9bc940d8-7dfb-4950-98f4-6853f398cb49
relation.isAuthorOfPublicationd354b937-b18c-4ca5-a843-04a04269d60f
relation.isAuthorOfPublication.latestForDiscoveryd354b937-b18c-4ca5-a843-04a04269d60f
relation.isProjectOfPublicationfafa76a6-2cd2-4a6d-a3c9-772f34d3b91f
relation.isProjectOfPublication15f91d45-e070-47d8-b6b8-efd4de31d9a8
relation.isProjectOfPublication.latestForDiscoveryfafa76a6-2cd2-4a6d-a3c9-772f34d3b91f

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Role of the melanocortin system in zebrafish skin physiology.pdf
Size:
5.69 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.46 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)