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Pseudomonads from wild free-living sea turtles in Principe Island, Gulf of Guinea

2017-10Journal article Open access
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dc.contributor.authorOliveira, Manuela
dc.contributor.authorSerrano, Isa
dc.contributor.authorSantos, Jose Pedro
dc.contributor.authorBilocq, Florence
dc.contributor.authorPereira, Nuno
dc.contributor.authorLoureiro, Nuno de Santos
dc.contributor.authorTavares, Luis
dc.contributor.authorPirnay, Jean-Paul
dc.contributor.authorDe Vos, Daniel
dc.date.accessioned2019-11-20T15:07:17Z
dc.date.available2019-11-20T15:07:17Z
dc.date.issued2017-10
dc.description.abstractDissemination of antibiotic resistance is a major concern, especially in aquatic environments, where pollution contributes for resistant bacteria selection. These strains may have serious health implications, especially for endangered species, including the sea turtles' hawksbill Eretmochelys imbricata and green turtles Chelonia mydas. We aimed to evaluate the presence of antibiotic resistant pseudomonads in wild sea turtles from Principe Island, Sao Tome and Principe, Guinea Gulf. Isolates were obtained from oral and cloacal swabs of free-living turtles by conventional techniques. Pseudomonads screening was performed by multiplex-PCR (oprl/oprL) and biochemical identification and antibiotic resistance profiling were achieved using Vitek2. All pseudomonad isolates were genotyped by Rep-PCR. Thirteen isolates were oprl-positive and classified as pseudomonads, eight from the genus Pseudomonas with the species P. aeruginosa, P. stutzeri, and P. mendocina, and five co-isolated Alcaligenes faecalis. The P. aeruginosa isolate was also oprL-positive. Regarding isolates susceptibility profile, 38.5% were susceptible to all antibiotics tested, and multidrug resistant (MDR) strains were not identified. DNA fingerprinting did not show any specific clonal-cluster similarity. Data on the worldwide incidence of antibiotic resistance among wildlife is still very scarce, especially concerning remote tropical areas. Since Pseudomonas genus has emerged as a group of increasingly reported opportunistic microorganisms in human and veterinary medicine with high resistance levels, it could be used as a tool for environmental resistance surveillance, particularly considering their ubiquity.
dc.description.sponsorshipOceandrio de Lisboa, Portugal
dc.description.sponsorshipMarine Turtle Conservation Act - U.S. Fish & Wildlife Service grant
dc.description.sponsorshipInterdisciplinary Research Centre for Animal Health, Faculty of Veterinary Medicine, University of Lisbon (FMV/UL) [UID/CVT/00276/2013]
dc.description.sponsorshipLaboratory of Molecular and Cellular Technology, Queen Astrid Military Hospital, Brussels, Belgium
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1016/j.ecolind.2017.06.005
dc.identifier.issn1470-160X
dc.identifier.issn1872-7034
dc.identifier.urihttp://hdl.handle.net/10400.1/12963
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.subjectPopulation-structure
dc.subjectAntimicrobial resistance
dc.subjectChelonia-mydas
dc.subjectAeruginosa
dc.subjectBacteria
dc.subjectConsumption
dc.subjectTortoise
dc.titlePseudomonads from wild free-living sea turtles in Principe Island, Gulf of Guinea
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FCVT%2F00276%2F2013/PT
oaire.citation.endPage264
oaire.citation.startPage260
oaire.citation.titleEcological Indicators
oaire.citation.volume81
oaire.fundingStream5876
person.familyNameFigueiredo de Santos Loureiro
person.givenNameNuno Manuel de
person.identifier.ciencia-idD514-3F01-6DE0
person.identifier.orcid0000-0001-7018-7630
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
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relation.isAuthorOfPublication.latestForDiscoverya281c547-4ec4-4512-ba80-350af27857cf
relation.isProjectOfPublicationb45dea40-0b18-4fba-9f3d-c5feff8289c9
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