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Molecular richness and biotechnological potential of bacteria cultured from Irciniidae sponges in the north-east Atlantic

2013-09Journal article Open access
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dc.contributor.authorEsteves, Ana
dc.contributor.authorHardoim, Cristiane
dc.contributor.authorXavier, Joana R.
dc.contributor.authorGonçalves, Jorge Manuel Santos
dc.contributor.authorCosta, Rodrigo
dc.date.accessioned2018-12-07T14:58:13Z
dc.date.available2018-12-07T14:58:13Z
dc.date.issued2013-09
dc.description.abstractSeveral bioactive compounds originally isolated from marine sponges have been later ascribed or suggested to be synthesized by their symbionts. The cultivation of sponge-associated bacteria provides one possible route to the discovery of these metabolites. Here, we determine the bacterial richness cultured from two irciniid sponge species, Sarcotragus spinosulus and Ircinia variabilis, and ascertain their biotechnological potential. A total of 279 isolates were identified from 13 sponge specimens. These were classified into 17 genera - with Pseudovibrio, Ruegeria and Vibrio as the most dominant - and 3 to 10 putatively new bacterial species. While 16S rRNA gene sequencing identified 29 bacterial phylotypes at the 'species' level (97% sequence similarity), whole-genome BOX-PCR fingerprinting uncovered 155 genotypes, unveiling patterns of specimen-dependent occurrence of prevailing bacterial genomes across sponge individuals. Among the BOX-PCR genotypes recovered, 34% were active against clinically relevant strains, with Vibrio isolates producing the most active antagonistic effect. Several Pseudovibrio genotypes showed the presence of polyketide synthase (PKS) genes, and these were for the first time detected in isolates of the genus Aquimarina (Bacteroidetes). Our results highlight great biotechnological potential and interest for the Irciniidae sponge family and their diversified bacterial genomes.
dc.description.sponsorshipPortuguese Foundation for Science and Technology (FCT) [PTDC/MAR/101431/2008]; FCT [SFRH/BD/60873/2009, SFRH/BPD/62946/2009]
dc.identifier.doi10.1111/1574-6941.12140
dc.identifier.issn0168-6496
dc.identifier.issn1574-6941
dc.identifier.urihttp://hdl.handle.net/10400.1/11915
dc.language.isoeng
dc.peerreviewedyes
dc.publisherOxford Univ Press
dc.subjectChina sea sponges
dc.subjectMarine sponges
dc.subjectAntimicrobial activities
dc.subjectPolyketide biosynthesis
dc.subjectPhylogenetic diversity
dc.subjectMediterranean sponges
dc.subjectVertical transmission
dc.subjectMicrobial community
dc.subjectElectron microscope
dc.subjectAplysina aerophoba
dc.titleMolecular richness and biotechnological potential of bacteria cultured from Irciniidae sponges in the north-east Atlantic
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR%2F101431%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F60873%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F62946%2F2009/PT
oaire.citation.endPage536
oaire.citation.issue3
oaire.citation.startPage519
oaire.citation.titleFems Microbiology Ecology
oaire.citation.volume85
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
oaire.fundingStreamSFRH
person.familyNameEsteves
person.familyNameHardoim
person.familyNameGonçalves
person.familyNameda Silva Costa
person.givenNameAna
person.givenNameCristiane
person.givenNameJorge Manuel Santos
person.givenNameRodrigo
person.identifier293165
person.identifier356846
person.identifier115920
person.identifier.ciencia-id1013-ED6A-65EB
person.identifier.ciencia-id5917-D500-D251
person.identifier.orcid0000-0002-9614-0635
person.identifier.orcid0000-0003-4650-0177
person.identifier.orcid0000-0001-7704-8190
person.identifier.orcid0000-0002-5932-4101
person.identifier.ridD-4544-2015
person.identifier.ridG-5206-2011
person.identifier.ridN-7274-2013
person.identifier.scopus-author-id22950697100
person.identifier.scopus-author-id7103326116
person.identifier.scopus-author-id7203063627
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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