Repository logo
 
Publication

PTHrP-induced modifications of the sea bream (Sparus auratus) vertebral bone proteome

2013-09-15Journal article Open Access
Simple item page
dc.contributor.authorAnjos, Liliana
dc.contributor.authorRedruello, Begoña
dc.contributor.authorReinhardt, Richard
dc.contributor.authorCanario, Adelino V. M.
dc.contributor.authorPower, Deborah
dc.date.accessioned2014-05-13T12:54:52Z
dc.date.available2014-05-13T12:54:52Z
dc.date.available2015-06-01
dc.date.issued2013-09-15
dc.date.updated2014-05-13T08:33:31Z
dc.description.abstractEndocrine factors play an essential role in the formation and turnover of the skeleton in vertebrates. In the present study sea bream vertebral bone transcripts for PTH1R and PTH3R were identified and the action of intermittent administration of parathyroid hormone related protein (PTHrP) on the proteome of vertebral bone was analysed. Treatment of immature sea bream (Sparus auratus, n = 6) for 5 days with homologous recombinant PTHrP(1–125; 150 ng/g body weight) modified bone metabolism and caused a significant (p < 0.05) reduction in both tartrate resistant acid phosphatase (TRACP) and alkaline phosphatase (ALP) in relation to control fish. However, the ratio of TRACP: ALP in PTHrP treated fish (1.3 to 2.2 cf. control) suggested it had an anabolic response. A sea bream vertebral bone proteome of 157 protein spots was generated and putative identity assigned to 118 (75.2%) proteins of which 72% had homology to proteins/transcripts from teleosts many of which have not previously been reported in teleost bone. Classification of bone proteins using gene ontology revealed those with protein or metal/ion (e.g., calcium, magnesium, zinc) binding (∼53%) activities were most abundant. The expression of eight proteins was significantly (p < 0.05) modified in the vertebra of PTHrP treated compared to control fish; three were up-regulated, betainehomocystein S-methyltransferase, glial fibrillary acidic protein, parvalbumin beta and five were down-regulated, annexin A5, apolipoprotein A1, myosin light chain 2, fast skeletal myosin light chain 3, troponin C. In conclusion, intermittent administration of PTHrP to sea bream is associated with an anabolic response in vertebral bone metabolism and modifies calcium binding proteins in the proteome.por
dc.identifier.citationAnjos, Liliana; Gomes, Ana S.; Redruello, Begoña; Reinhardt, Richard; Canário, Adelino V.M.; Power, Deborah M.PTHrP-induced modifications of the sea bream (Sparus auratus) vertebral bone proteome, General and Comparative Endocrinology, 191, Jun. 2013, 102-112, 2013.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.ygcen.2013.05.014
dc.identifier.issn0016-6480
dc.identifier.otherAUT: ACA00258; DPO00386
dc.identifier.urihttp://hdl.handle.net/10400.1/3903
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationBuilding a biological knowledge-base on fish lifecycles for competitive, sustainable European aquaculture
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0016648013002529por
dc.subjectTeleost bone proteomepor
dc.subjectCalcium binding proteinspor
dc.subjectAnabolismpor
dc.subjectSea bream recombinant parathyroid hormone related peptidepor
dc.subjectCalcium binding proteinspor
dc.subjectTeleost bone proteomepor
dc.titlePTHrP-induced modifications of the sea bream (Sparus auratus) vertebral bone proteomepor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBuilding a biological knowledge-base on fish lifecycles for competitive, sustainable European aquaculture
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/222719/EU
oaire.citation.endPage112por
oaire.citation.startPage102por
oaire.citation.titleGeneral and Comparative Endocrinologypor
oaire.citation.volume191por
oaire.fundingStreamFP7
person.familyNameAnjos Guerreiro
person.familyNameRedruello
person.familyNameCanario
person.familyNamePower
person.givenNameLiliana Isabel Tomé
person.givenNameBegoña
person.givenNameAdelino
person.givenNameDeborah Mary
person.identifierAAK-3800-2020
person.identifier143624
person.identifier.ciencia-id6D18-2D1C-A45C
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0001-9474-522X
person.identifier.orcid0000-0003-1787-5594
person.identifier.orcid0000-0002-6244-6468
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id8575757700
person.identifier.scopus-author-id56568523700
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication721b5dca-ad42-40f8-9731-517eb5db009e
relation.isAuthorOfPublication67798ebb-16f5-4974-8d52-5158ddf9fe53
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscovery721b5dca-ad42-40f8-9731-517eb5db009e
relation.isProjectOfPublicationeb95e695-02d8-41f1-88df-951429f0f4f3
relation.isProjectOfPublication.latestForDiscoveryeb95e695-02d8-41f1-88df-951429f0f4f3

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
PTHrP-induced modifications of the sea bream (Sparus auratus) vertebral bone proteome.pdf
Size:
1.26 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.61 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)