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Enzymatic capacities of metabolic fuel use in cuttlefish (Sepia officinalis) and responses to food deprivation: insight into the metabolic organization and starvation survival strategy of cephalopods

2016-08Journal article Open access
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dc.contributor.authorSpeers-Roesch, Ben
dc.contributor.authorCallaghan, Neal I.
dc.contributor.authorMacCormack, Tyson J.
dc.contributor.authorLamarre, Simon G.
dc.contributor.authorSykes, António
dc.contributor.authorDriedzic, William R.
dc.date.accessioned2017-04-07T15:56:20Z
dc.date.available2017-04-07T15:56:20Z
dc.date.issued2016-08
dc.description.abstractFood limitation is a common challenge for animals. Cephalopods are sensitive to starvation because of high metabolic rates and growth rates related to their "live fast, die young" life history. We investigated how enzymatic capacities of key metabolic pathways are modulated during starvation in the common cuttlefish (Sepia officinalis) to gain insight into the metabolic organization of cephalopods and their strategies for coping with food limitation. In particular, lipids have traditionally been considered unimportant fuels in cephalopods, yet, puzzlingly, many species (including cuttlefish) mobilize the lipid stores in their digestive gland during starvation. Using a comprehensive multi-tissue assay of enzymatic capacities for energy metabolism, we show that, during long-term starvation (12 days), glycolytic capacity for glucose use is decreased in cuttlefish tissues, while capacities for use of lipid-based fuels (fatty acids and ketone bodies) and amino acid fuels are retained or increased. Specifically, the capacity to use the ketone body acetoacetate as fuel is widespread across tissues and gill has a previously unrecognized capacity for fatty acid catabolism, albeit at low rates. The capacity for de novo glucose synthesis (gluconeogenesis), important for glucose homeostasis, likely is restricted to the digestive gland, contrary to previous reports of widespread gluconeogenesis among cephalopod tissues. Short-term starvation (3-5 days) had few effects on enzymatic capacities. Similar to vertebrates, lipid-based fuels, putatively mobilized from fat stores in the digestive gland, appear to be important energy sources for cephalopods, especially during starvation when glycolytic capacity is decreased perhaps to conserve available glucose.
dc.description.sponsorshipSEPIATECH 31-03-05-FEP-2 PROMAR
dc.identifier.doi10.1007/s00360-016-0991-3
dc.identifier.issn0174-1578
dc.identifier.urihttp://hdl.handle.net/10400.1/9384
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer Verlag
dc.relationTowards cephalopod aquaculture: cuttlefish as the spearhead species
dc.relation.isbasedonWOS:000379326000004
dc.subjectCephalopod
dc.subjectEnergy metabolism
dc.subjectMetabolic fuel preference
dc.subjectEnzyme activity
dc.subjectStarvation
dc.subjectFasting
dc.subjectLipid
dc.subjectKetone body
dc.subjectAmino acid
dc.titleEnzymatic capacities of metabolic fuel use in cuttlefish (Sepia officinalis) and responses to food deprivation: insight into the metabolic organization and starvation survival strategy of cephalopods
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTowards cephalopod aquaculture: cuttlefish as the spearhead species
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00576%2F2014%2FCP1217%2FCT0002/PT
oaire.citation.endPage725
oaire.citation.issue6
oaire.citation.startPage711
oaire.citation.titleJournal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology
oaire.citation.volume186
oaire.fundingStreamInvestigador FCT
person.familyNameSykes
person.givenNameAntónio
person.identifier107454
person.identifier.ciencia-id7510-6641-5A42
person.identifier.orcid0000-0002-5207-0612
person.identifier.ridC-3609-2012
person.identifier.scopus-author-id7102626426
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
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relation.isAuthorOfPublication.latestForDiscoveryb9d55f95-5de8-40cf-82a3-96ec98c91a55
relation.isProjectOfPublicationa7bbb7aa-912c-4d7f-933a-3905800d8dcf
relation.isProjectOfPublication.latestForDiscoverya7bbb7aa-912c-4d7f-933a-3905800d8dcf

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