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Retinoic acid differentially affects in vitro proliferation, differentiation and mineralization of two fish bone-derived cell lines: Different gene expression of nuclear receptors and ECM proteins

dc.contributor.authorFernández, Ignacio
dc.contributor.authorTiago, Daniel
dc.contributor.authorLaizé, Vincent
dc.contributor.authorCancela, Leonor
dc.contributor.authorGisbert, Enric
dc.date.accessioned2014-06-09T13:58:25Z
dc.date.available2014-06-09T13:58:25Z
dc.date.issued2014-03
dc.date.updated2014-06-03T09:33:15Z
dc.description.abstractRetinoic acid (RA), the main active metabolite of vitamin A, regulates vertebrate morphogenesis through signaling pathways not yet fully understood. Such process involves the specific activation of retinoic acid and retinoid X receptors (RARs and RXRs), which are nuclear receptors of the steroid/thyroid hormone receptor superfamily. Teleost fish are suitable models to study vertebrate development, such as skeletogenesis. Cell systems capable of in vitro mineralization have been developed for several fish species and may provide new insights into the specific cellular and molecular events related to vitamin A activity in bone, complementary to in vivo studies. This work aims at investigating the in vitro effects of RA (0.5 and 12.5 μM) on proliferation, differentiation and extracellular matrix (ECM) mineralization of two gilthead seabream bone-derived cell lines (VSa13 and VSa16), and at identifying molecular targets of its action through gene expression analysis. RA induced phenotypic changes and cellular proliferation was inhibited in both cell lines in a cell type-dependent manner (36–59% in VSa13 and 17–46% in VSa16 cells). While RA stimulated mineral deposition in VSa13 cell cultures (50–62% stimulation), it inhibited the mineralization of extracellular matrix in VSa16 cells (11–57% inhibition). Expression of hormone receptor genes (rars and rxrs), and extracellular matrix-related genes such as matrix and bone Gla proteins (mgp and bglap), osteopontin (spp1) and type I collagen (col1a1) were differentially regulated upon exposure to RA in proliferating, differentiating and mineralizing cultures of VSa13 and VSa16 cells. Altogether, our results show: (i) RA affects proliferative and mineralogenic activities in two fish skeletal cell types and (ii) that during phenotype transitions, specific RA nuclear receptors and bone-related genes are differentially expressed in a cell type-dependent manner.por
dc.identifier.citationFernández, Ignacio; Tiago, Daniel M; Laizé, Vincent; Leonor Cancela, M.; Gisbert, Enric. Retinoic acid differentially affects in vitro proliferation, differentiation and mineralization of two fish bone-derived cell lines: Different gene expression of nuclear receptors and ECM proteins, The Journal of Steroid Biochemistry and Molecular Biology, 140, 34-43, 2014.por
dc.identifier.doihttp://dx.doi.org/ 10.1016/j.jsbmb.2013.11.012
dc.identifier.issn0960-0760
dc.identifier.otherAUT: LCA00739;
dc.identifier.urihttp://hdl.handle.net/10400.1/4258
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0960076013002677por
dc.subjectRetinoic acidpor
dc.subjectGene expressionpor
dc.subjectBone-derived cell linespor
dc.subjectChondrocytepor
dc.subjectOsteoblastpor
dc.subjectVitamin Apor
dc.subjectGilthead seabream Sparus auratapor
dc.titleRetinoic acid differentially affects in vitro proliferation, differentiation and mineralization of two fish bone-derived cell lines: Different gene expression of nuclear receptors and ECM proteinspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage43por
oaire.citation.startPage34por
oaire.citation.titleThe Journal of Steroid Biochemistry and Molecular Biologypor
oaire.citation.volume140por
person.familyNameTiago
person.familyNameLaizé
person.familyNameCancela
person.familyNameGisbert
person.givenNameDaniel
person.givenNameVincent
person.givenNameM. Leonor
person.givenNameEnric
person.identifier606918
person.identifier.orcid0000-0001-8418-6292
person.identifier.orcid0000-0001-9565-9198
person.identifier.orcid0000-0003-3114-6662
person.identifier.orcid0000-0002-7457-8468
person.identifier.ridB-4463-2008
person.identifier.ridB-3654-2011
person.identifier.scopus-author-id14422711000
person.identifier.scopus-author-id6602982778
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublicationb51cd3d5-7ee4-47d8-9bc4-b3e297aa222f
relation.isAuthorOfPublicationb9bbfe32-3dfe-4131-ad14-a4394008447f
relation.isAuthorOfPublicationd45a0129-65f8-469d-a8d9-74c1d8c40284
relation.isAuthorOfPublication.latestForDiscovery10cda3a0-7b9c-4d16-bd2a-a06df1d56d94

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